• Featured-Product
ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)

IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 1 (IGFBP1).
No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 1 (IGFBP1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Insulin Like Growth Factor Binding Protein 1 (IGFBP1) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin Like Growth Factor Binding Protein 1 (IGFBP1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-96 87
EDTA plasma(n=5) 79-89 78
heparin plasma(n=5) 86-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 1 (IGFBP1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Like Growth Factor Binding Protein 1 (IGFBP1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin Like Growth Factor Binding Protein 1 (IGFBP1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-104% 78-104% 78-91% 78-91%
EDTA plasma(n=5) 88-101% 90-98% 98-105% 96-104%
heparin plasma(n=5) 79-89% 90-99% 90-105% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Endocrinology Lack of Dietary Carbohydrates Induces Hepatic Growth Hormone (GH) Resistance in Rats Endo: source
Endocrinology Serum IGF-I Is Not a Reliable Pharmacodynamic Marker of Exogenous Growth Hormone Activity in Mice Endo: source
Animal. Effects of a dietary crude fibre concentrate on growth in weaned piglets. pubmed:28318464
Stem Cell Research & Therapy Differentiation of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells into endometrial cells pubmed:29096715
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA052Hu01 Recombinant Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA052Hu02 Recombinant Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Positive Control; Immunogen; SDS-PAGE; WB.
EPA052Hu61 Eukaryotic Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Positive Control; Immunogen; SDS-PAGE; WB.
PAA052Hu01 Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC; IP.
PAA052Hu02 Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC; IP.
LAA052Hu81 FITC-Linked Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC; IF.
LAA052Hu71 Biotin-Linked Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC.
MAA052Hu24 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC; IP.
MAA052Hu25 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC; IP.
MAA052Hu22 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB,IHC
MAA052Hu21 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB,IHC
MAA052Hu23 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB
MAA052Hu27 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; IF
MAA052Hu26 Monoclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) WB; IHC; ICC; IP.
FAA052Hu08 Anti-Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Monoclonal Antibody Flow cytometry.
FAA052Hu02 Anti-Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Monoclonal Antibody Flow cytometry.
FAA052Hu04 Anti-Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Monoclonal Antibody Flow cytometry.
FAA052Hu06 Anti-Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Monoclonal Antibody Flow cytometry.
SEA052Hu ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA052Hu CLIA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Chemiluminescent immunoassay for Antigen Detection.
LMA052Hu Multiplex Assay Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA052Hu01 ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Main materials for "Do It (ELISA Kit) Yourself".