ELISA Kit for Interleukin 8 (IL8)
CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1
- Product No.SEA080Hu
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range15.6-1,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 6.7pg/mL.
- DownloadInstruction Manual
Ensure in stock; if not, free redo!
- FOB US$ 321
For negotiated price and more details, please contact local distributors! US$ 458
For negotiated price and more details, please contact local distributors! US$ 2061
For negotiated price and more details, please contact local distributors! US$ 3893
For negotiated price and more details, please contact local distributors! US$ 32060
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Acta Otolaryngol||Serial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244|
|Journal of Clinical Periodontology||Experimental peri-implant mucositis at different implant surfaces.Pubmed: 24521508|
|Rheumatology International||Serum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186|
|J Med Life.||Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer disseminationPubmed:Pmc4197484|
|J Breath Res||Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610|
|ARTICLE||The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in EgyptProfile: Mohmad_Mosaad|
|Veterinary Immunology and Immunopathology||Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodPubmed:27090626|
|Archives of Biological Sciences||Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu|
|journal of molecular and cellular cardiology||Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.pubmed:28088561|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications|
|APA080Hu01||Active Interleukin 8 (IL8)||Cell culture; Activity Assays.|
|RPA080Hu01||Recombinant Interleukin 8 (IL8)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA080Hu01||Polyclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC; IP.|
|LAA080Hu71||Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC.|
|LAA080Hu81||FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC; IF.|
|MAA080Hu24||Monoclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC; IP.|
|MAA080Hu22||Monoclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC; IP.|
|LAA080Hu82||FITC-Linked Monoclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC.|
|LAA080Hu72||Biotin-Linked Monoclonal Antibody to Interleukin 8 (IL8)||WB; IHC; ICC.|
|SEA080Hu||ELISA Kit for Interleukin 8 (IL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|HEA080Hu||High Sensitive ELISA Kit for Interleukin 8 (IL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|IEA080Hu||Instant ELISA Kit for Interleukin 8 (IL8)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA080Hu||CLIA Kit for Interleukin 8 (IL8)||Chemiluminescent immunoassay for Antigen Detection.|
|PSA080Hu01||Antibody Pair for Interleukin 8 (IL8)||ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.|
|KSA080Hu01||ELISA Kit DIY Materials for Interleukin 8 (IL8)||Main materials for "Do It (ELISA Kit) Yourself".|