ELISA Kit for Interleukin 8 (IL8)

CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 8 (IL8) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 8 (IL8) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)88-10296
EDTA plasma(n=5)79-9187
heparin plasma(n=5)97-104101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 8 (IL8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)91-101%79-93%97-105%95-103%
EDTA plasma(n=5)93-101%86-103%80-91%82-103%
heparin plasma(n=5)80-101%81-93%78-93%96-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

MagazineReference
Acta OtolaryngolSerial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244
Journal of Clinical PeriodontologyExperimental peri-implant mucositis at different implant surfaces.Pubmed: 24521508
Rheumatology InternationalSerum levels of selected chemokines in systemic lupus erythematosus patientsPubmed: 22461186
J Med Life.Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer disseminationPubmed:Pmc4197484
J Breath ResEffects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgeryPubMed: 25719610
ARTICLEThe Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in EgyptProfile: Mohmad_Mosaad
Veterinary Immunology and ImmunopathologyNeutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation periodPubmed:27090626
Archives of Biological SciencesDiabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrolpublication:299492265_Diabetes-induced_renal_failu
journal of molecular and cellular cardiologyMutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.pubmed:28088561
中国药物警戒小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响Periodical_zgywjj201608004
Catalog No.Related products for research use of Homo sapiens (Human) Organism speciesApplications
APA080Hu01Active Interleukin 8 (IL8)Cell culture; Activity Assays.
RPA080Hu01Recombinant Interleukin 8 (IL8)Positive Control; Immunogen; SDS-PAGE; WB.
PAA080Hu01Polyclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IP.
LAA080Hu71Biotin-Linked Polyclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC.
LAA080Hu81FITC-Linked Polyclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IF.
MAA080Hu24Monoclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IP.
MAA080Hu22Monoclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC; IP.
LAA080Hu82FITC-Linked Monoclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC.
LAA080Hu72Biotin-Linked Monoclonal Antibody to Interleukin 8 (IL8)WB; IHC; ICC.
SEA080HuELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection.
HEA080HuHigh Sensitive ELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection.
IEA080HuInstant ELISA Kit for Interleukin 8 (IL8)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA080HuCLIA Kit for Interleukin 8 (IL8)Chemiluminescent immunoassay for Antigen Detection.
PSA080Hu01Antibody Pair for Interleukin 8 (IL8)ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA080Hu01ELISA Kit DIY Materials for Interleukin 8 (IL8)Main materials for "Do It (ELISA Kit) Yourself".