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ELISA Kit for Matrix Metalloproteinase 2 (MMP2)

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

Specificity

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 2 (MMP2).
No significant cross-reactivity or interference between Matrix Metalloproteinase 2 (MMP2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 2 (MMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 2 (MMP2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 93
heparin plasma(n=5) 87-102 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 2 (MMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 89-101% 88-95% 82-91%
heparin plasma(n=5) 98-105% 84-101% 87-101% 85-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Moleculer Vision Bone morphogenetic protein-2: a potential regulator in scleral remodeling PubMed: PMC2605409
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus Pubmed: source
Zahedan Journal of Research in Medical Sciences The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men Zjrms: Source
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
Experimental & Translational Stroke Medicine Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435
PLoS One Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Clinical and Investigative Medicine The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells Pubmed: 23544611
Physiol. Res. The unwounded skin remodeling in animal models of diabetes types 1 and 2. Pubmed: 24020818
Acta Histochemica RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells Pubmed: 24681113
Peritoneal Dialysis Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients? Pubmed: 23994841
Polski Przeglad Chirurgiczny Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Int Immunopharmacol.? Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis Pubmed:25824718
Journal of Perinatology Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion Pubmed:25078863
Biomedical Materials In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study Pubmed:25634132
Stem Cells International Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Pubmed:25767513
Physiology and Pharmacology A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men Phypha:Source
Mediators of Inflammation Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi
Journal of Biomaterials and Tissue Engineering MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 Content: Asp
Biomedical Materials In vivo PubMed: 25634132
Int J Clin Exp Pathol Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways PubMed: 26191138
Hattori and Ishihara Stem Cell Research & Therapy Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells PubMed: 25884474
Biosens Bioelectron Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+ PubMed: 26433066
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140
Canadian Journal of Physiology and Pharmacology Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats Doi: Abs
PLASTIC AND AESTHETIC RESEARCH A comparative study to evaluate the effect of limited access dressing on diabetic ulcers Article: 2347-9264
Int J Mol Sci Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ PubMed: 26703570
Basic Research CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699
International Journal of Oncology 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway Pubmed:26782341
International Journal of Pharmacology Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration profile:57162df608aebf0697f1843d
Journal of Stroke and Cerebrovascular Diseases Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats Pubmed:26851971
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection Pubmed:26925446
Cell Physiol Biochem. MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 Pubmed:27160777
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
Biosensors and Bioelectronics Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates science:S0956566315304474
world journal of surgery Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia. pubmed:28050662
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451
Balkan Medical Journal Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage pubmed:27761275
Microvascular Research Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518
BMC Veterinary Research Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355
Cellular Physiology and Biochemistry MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. pubmed:27160777
Journal of Biomaterials and Tissue Engineering MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line publication:306067745
World Journal of Plastic Surgery Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings. pubmed:27853690
Journal of Biomaterials and Tissue Engineering Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts art00008
Human cell Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity pubmed:28091828
Human Cell MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 pubmed:27743351
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