ELISA Kit for Matrix Metalloproteinase 2 (MMP2)
- Promotion products
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
- Product No.SEA100Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range31.2-2000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 12.1pg/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
Ensure in stock; if not, free redo!
For negotiated price and more details, please contact local distributors! US$ 419
For negotiated price and more details, please contact local distributors! US$ 1886
For negotiated price and more details, please contact local distributors! US$ 3562
For negotiated price and more details, please contact local distributors! US$ 29330
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 2 (MMP2).
No significant cross-reactivity or interference between Matrix Metalloproteinase 2 (MMP2) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 2 (MMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 2 (MMP2) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 2 (MMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Moleculer Vision||Bone morphogenetic protein-2: a potential regulator in scleral remodeling PubMed: PMC2605409|
|Neuroimmunomodulation||Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus Pubmed: source|
|Zahedan Journal of Research in Medical Sciences||The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men Zjrms: Source|
|Experimental Biology and Medicine||Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source|
|Experimental & Translational Stroke Medicine||Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435|
|PLoS One||Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697|
|PLoS ONE||A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source|
|Clinical and Investigative Medicine||The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells Pubmed: 23544611|
|Physiol. Res.||The unwounded skin remodeling in animal models of diabetes types 1 and 2. Pubmed: 24020818|
|Acta Histochemica||RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells Pubmed: 24681113|
|Peritoneal Dialysis||Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients? Pubmed: 23994841|
|Polski Przeglad Chirurgiczny||Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206|
|Thromb Haemost.||Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655|
|Int Immunopharmacol.?||Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis Pubmed:25824718|
|Journal of Perinatology||Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion Pubmed:25078863|
|Biomedical Materials||In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study Pubmed:25634132|
|Stem Cells International||Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Pubmed:25767513|
|Physiology and Pharmacology||A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men Phypha:Source|
|Mediators of Inflammation||Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi|
|Journal of Biomaterials and Tissue Engineering||MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 Content: Asp|
|Biomedical Materials||In vivo PubMed: 25634132|
|Int J Clin Exp Pathol||Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways PubMed: 26191138|
|Hattori and Ishihara Stem Cell Research & Therapy||Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells PubMed: 25884474|
|Biosens Bioelectron||Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+ PubMed: 26433066|
|Veterinary Parasitology||Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140|
|Canadian Journal of Physiology and Pharmacology||Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats Doi: Abs|
|PLASTIC AND AESTHETIC RESEARCH||A comparative study to evaluate the effect of limited access dressing on diabetic ulcers Article: 2347-9264|
|Int J Mol Sci||Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ PubMed: 26703570|
|Basic Research||CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699|
|International Journal of Oncology||1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway Pubmed:26782341|
|International Journal of Pharmacology||Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration profile:57162df608aebf0697f1843d|
|Journal of Stroke and Cerebrovascular Diseases||Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats Pubmed:26851971|
|VirusDisease||Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection Pubmed:26925446|
|Cell Physiol Biochem.||MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 Pubmed:27160777|
|Int Wound J.||From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748|
|Biosensors and Bioelectronics||Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates science:S0956566315304474|
|world journal of surgery||Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia. pubmed:28050662|
|disease markers||Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451|
|Balkan Medical Journal||Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage pubmed:27761275|
|Microvascular Research||Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518|
|BMC Veterinary Research||Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355|
|Cellular Physiology and Biochemistry||MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. pubmed:27160777|
|Journal of Biomaterials and Tissue Engineering||MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line publication:306067745|
|World Journal of Plastic Surgery||Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings. pubmed:27853690|
|Journal of Biomaterials and Tissue Engineering||Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts art00008|
|Human cell||Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity pubmed:28091828|
|Human Cell||MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 pubmed:27743351|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA100Hu01||Recombinant Matrix Metalloproteinase 2 (MMP2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA100Hu01||Active Matrix Metalloproteinase 2 (MMP2)||Cell culture; Activity Assays.|
|APA100Hu02||Active Matrix Metalloproteinase 2 (MMP2)||Cell culture; Activity Assays.|
|RPA100Hu02||Recombinant Matrix Metalloproteinase 2 (MMP2)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA100Hu02||Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2)||WB; IHC; ICC; IP.|
|PAA100Hu01||Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2)||WB; IHC; ICC; IP.|
|LAA100Hu71||Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2)||WB; IHC; ICC.|
|LAA100Hu81||FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2)||WB; IHC; ICC; IF.|
|MAA100Hu22||Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2)||WB; IHC; ICC; IP.|
|SEA100Hu||ELISA Kit for Matrix Metalloproteinase 2 (MMP2)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA100Hu||CLIA Kit for Matrix Metalloproteinase 2 (MMP2)||Chemiluminescent immunoassay for Antigen Detection.|