• Featured-Product
ELISA Kit for Matrix Metalloproteinase 2 (MMP2)

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

Specificity

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 2 (MMP2).
No significant cross-reactivity or interference between Matrix Metalloproteinase 2 (MMP2) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Matrix Metalloproteinase 2 (MMP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Matrix Metalloproteinase 2 (MMP2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-98 93
heparin plasma(n=5) 87-102 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 2 (MMP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Matrix Metalloproteinase 2 (MMP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 89-101% 88-95% 82-91%
heparin plasma(n=5) 98-105% 84-101% 87-101% 85-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Citations
Moleculer Vision Bone morphogenetic protein-2: a potential regulator in scleral remodeling PubMed: PMC2605409
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus Pubmed: source
Zahedan Journal of Research in Medical Sciences The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men Zjrms: Source
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
Experimental & Translational Stroke Medicine Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke PubMed: 21679435
PLoS One Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 PubMed: PMC3797697
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Clinical and Investigative Medicine The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells Pubmed: 23544611
Physiol. Res. The unwounded skin remodeling in animal models of diabetes types 1 and 2. Pubmed: 24020818
Acta Histochemica RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells Pubmed: 24681113
Peritoneal Dialysis Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients? Pubmed: 23994841
Polski Przeglad Chirurgiczny Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course Pubmed: 23585206
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke Pubmed:24671655
Int Immunopharmacol.? Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis Pubmed:25824718
Journal of Perinatology Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion Pubmed:25078863
Biomedical Materials In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study Pubmed:25634132
Stem Cells International Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Pubmed:25767513
Physiology and Pharmacology A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men Phypha:Source
Mediators of Inflammation Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse Journals: Mi
Journal of Biomaterials and Tissue Engineering MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 Content: Asp
Biomedical Materials In vivo PubMed: 25634132
Int J Clin Exp Pathol Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways PubMed: 26191138
Hattori and Ishihara Stem Cell Research & Therapy Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells PubMed: 25884474
Biosens Bioelectron Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+ PubMed: 26433066
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis PubMed: 26412140
Canadian Journal of Physiology and Pharmacology Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats Doi: Abs
PLASTIC AND AESTHETIC RESEARCH A comparative study to evaluate the effect of limited access dressing on diabetic ulcers Article: 2347-9264
Int J Mol Sci Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ PubMed: 26703570
Basic Research CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma Journal:27699
International Journal of Oncology 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway Pubmed:26782341
International Journal of Pharmacology Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration profile:57162df608aebf0697f1843d
Journal of Stroke and Cerebrovascular Diseases Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats Pubmed:26851971
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection Pubmed:26925446
Cell Physiol Biochem. MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 Pubmed:27160777
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
Biosensors and Bioelectronics Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates science:S0956566315304474
world journal of surgery Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia. pubmed:28050662
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. pubmed:28348451
Balkan Medical Journal Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage pubmed:27761275
Microvascular Research Pleiotropic effects of survivin in vascular endothelial cells. pubmed:27373518
BMC Veterinary Research Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. pubmed:27938355
Cellular Physiology and Biochemistry MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. pubmed:27160777
Journal of Biomaterials and Tissue Engineering MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line publication:306067745
World Journal of Plastic Surgery Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings. pubmed:27853690
Journal of Biomaterials and Tissue Engineering Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts art00008
Human cell Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity pubmed:28091828
Human Cell MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 pubmed:27743351
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis pubmed:28821422
Human Cell MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 pubmed:28573504
Cellular Physiology and Biochemistry The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells pubmed:29224019
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
Fundamental & Clinical Pharmacology Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 DOI:10.1111/fcp.12294
PLoS One Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955
Nanotoxicology Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway 10.1080/17435390.2017.1357213
Archives of Medical Science CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. pubmed:28507585
Molecular Medicine Reports Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis pubmed:28901438
European Journal of Medicinal Chemistry Syntenin-targeted peptide blocker inhibits progression of cancer cells Pubmed:29857221
Journal of Neurochemistry Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling Pubmed:29572844
Journal of Cellular Biochemistry Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia … 10.1002:jcb.26849
Inflammation Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy Pubmed:29704151
OncoTargets and Therapy sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion Pubmed:30022839
Biomed Research International CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling Pubmed:29984229
APMIS Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide Pubmed:30058214
Molecular Medicine Reports Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitro Pubmed:29328476
Oncology Letters miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells 10.3892:ol.2018.9170
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM Pubmed:29673663
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Pediatric Research Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. Pubmed: 30464333
Advanced Healthcare Materials Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts Pubmed: 30838809
Cellular & Molecular Biology Letters MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1
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Journal of endovascular therapy: an official journal of the International Society of Endovascular Specialists Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular … Pubmed: 31464166
Oncology Research MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 Pubmed: 31796150
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis Pubmed: 31980722
Acta Biomaterialia Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial Cells Pubmed: 32205213
JOURNAL OF THROMBOSIS AND THROMBOLYSIS Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension Pubmed: 33175289
JOURNAL OF ETHNOPHARMACOLOGY Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling … Pubmed: 32745509
heliyon Matrix levels of metalloproteinase-2 (MMP-2) and toxicity evaluation of carbonate apatite-based endodontic sealer in rat subcutaneous implantation Pubmed: 32671254
J Cell Physiol A novel role of VEPH1 in regulating AoSMC phenotypic switching Pubmed: 32342520
Advances in Animal and Veterinary Sciences Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats
Alteration on MMP-2 Levels in Women Diabetes Patients in Basrah
Veterinary Sciences: Research and Reviews Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep
MEDIATORS OF INFLAMMATION MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic 33488296
Oncol Lett GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression 33613720
Journal of Pregnancy Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia 33381317
Immunopharmacol Immunotoxicol Tofacitinib enhances remyelination and improves myelin integrity in cuprizone-induced mice 34618622
Exp Cell Res Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human … 34762897
Sci Rep Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026
Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer 34650982
Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study Pubmed:35743980
Journal of Pharmaceutical Research International Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
EPA100Hu62 Eukaryotic Matrix Metalloproteinase 2 (MMP2) Positive Control; Immunogen; SDS-PAGE; WB.
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PAA100Hu02 Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IP.
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LAA100Hu71 Biotin-Linked Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC.
LAA100Hu81 FITC-Linked Polyclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IF.
FAA100Hu41 PE-Linked Anti-Matrix Metalloproteinase 2 (MMP2) Polyclonal Antibody Flow cytometry.
MAA100Hu27 Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IP.
MAA100Hu23 Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IP.
MAA100Hu26 Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IP.
MAA100Hu22 Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC
MAA100Hu21 Monoclonal Antibody to Matrix Metalloproteinase 2 (MMP2) WB; IHC; ICC; IP.
FAA100Hu02 Anti-Matrix Metalloproteinase 2 (MMP2) Monoclonal Antibody Flow cytometry.
FAA100Hu04 Anti-Matrix Metalloproteinase 2 (MMP2) Monoclonal Antibody Flow cytometry.
FAA100Hu06 Anti-Matrix Metalloproteinase 2 (MMP2) Monoclonal Antibody Flow cytometry.
FAA100Hu08 Anti-Matrix Metalloproteinase 2 (MMP2) Monoclonal Antibody Flow cytometry.
SEA100Hu ELISA Kit for Matrix Metalloproteinase 2 (MMP2) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA100Hu CLIA Kit for Matrix Metalloproteinase 2 (MMP2) Chemiluminescent immunoassay for Antigen Detection.
LMA100Hu Multiplex Assay Kit for Matrix Metalloproteinase 2 (MMP2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.