ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)

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CD106; INCAM-100; L1CAM

Specificity

This assay has high sensitivity and excellent specificity for detection of Vascular Cell Adhesion Molecule 1 (VCAM1).
No significant cross-reactivity or interference between Vascular Cell Adhesion Molecule 1 (VCAM1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Vascular Cell Adhesion Molecule 1 (VCAM1) and the recovery rates were calculated by comparing the measured value to the expected amount of Vascular Cell Adhesion Molecule 1 (VCAM1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-99 95
EDTA plasma(n=5) 80-103 92
heparin plasma(n=5) 97-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vascular Cell Adhesion Molecule 1 (VCAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vascular Cell Adhesion Molecule 1 (VCAM1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-91% 95-103% 81-93% 79-90%
EDTA plasma(n=5) 98-105% 81-105% 99-105% 95-102%
heparin plasma(n=5) 85-96% 78-105% 80-103% 93-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
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Atherosclerosis Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro Pubmed: 24075742
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BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations Pubmed: 24199192
Arch Dis Child PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns Bmj:Source
Arch Dis Child? PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome Bmj:Source
Arch Dis Child PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography Bmj:Source
Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits Pubmed:25172523
Nutr Res. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits Pubmed:25282128
Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats Sciencedirect:S2090506814000268
Canadian Journal of Physiology and Pharmacology Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism Doi: Abs
Hum Exp Toxicol Effect of agmatine on experimental vascular endothelial dysfunction PubMed: 26424770
International Journal of Food Sciences and Nutrition Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products PubMed: 26401576
J Trace Elem Med Biol Comparative angioprotective effects of magnesium compounds PubMed: 25127069
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Mol Neurobiol A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats Pubmed:26867655
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