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ELISA Kit for Chemerin (CHEM)

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

Specificity

This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM).
No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-94 89
EDTA plasma(n=5) 81-95 88
heparin plasma(n=5) 89-102 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 86-104% 86-102% 95-105%
EDTA plasma(n=5) 83-97% 95-102% 80-90% 83-97%
heparin plasma(n=5) 89-96% 87-103% 86-101% 79-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

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Magazine Reference
Scandinavian Journal of Gastroenterology Serum chemerin and vaspin in non-alcoholic fatty liver disease PubMed: 20095887
Gastroenterology & Hepatology Chemerin, vaspin and insulin resistance in chronic hepatitis C Wiley: source
Placenta Expression and regulation of chemerin during rat pregnancy ScienceDirect: S0143400412000902
Acta Pharmacologica Sinica Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats Nature: 201211
RAED dergisi Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci Raeddergisi: Source
Journal of Cellular and Molecular Medicine Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients Pubmed: 24702860
Clinical Endocrinology Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects Pubmed: 24303851
Oral Dis. Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome Pubmed:24106991
Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia Pubmed:24840719
The Clinical Respiratory Journal Relationship between plasma chemerin levels and disease severity in COPD patients Pubmed:24865134
Gynecol Endocrinol. Elevated serum chemerin in Chinese women with hyperandrogenic PCOS Pubmed:24927079
Endocrine. Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. Pubmed:25471281
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Dermatologica Sinica Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris Sciencedirect:S1027811714000470
Dis Markers Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study PubMed: 26170530
Med Sci Monit Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population PubMed: 26471865
Endocrine Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats PubMed: 25471281
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes Pubmed:26721419
Medicine (Baltimore). Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen. pubmed:27583869
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA945Mu01 Recombinant Chemerin (CHEM) Positive Control; Immunogen; SDS-PAGE; WB.
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KSA945Mu01 ELISA Kit DIY Materials for Chemerin (CHEM) Main materials for "Do It (ELISA Kit) Yourself".