ELISA Kit for Chemerin (CHEM)
TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2
- Product No.SEA945Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.78 -50ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.32ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM).
No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Scandinavian Journal of Gastroenterology||Serum chemerin and vaspin in non-alcoholic fatty liver disease PubMed: 20095887|
|Gastroenterology & Hepatology||Chemerin, vaspin and insulin resistance in chronic hepatitis C Wiley: source|
|Placenta||Expression and regulation of chemerin during rat pregnancy ScienceDirect: S0143400412000902|
|Acta Pharmacologica Sinica||Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats Nature: 201211|
|RAED dergisi||Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci Raeddergisi: Source|
|Journal of Cellular and Molecular Medicine||Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients Pubmed: 24702860|
|Clinical Endocrinology||Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects Pubmed: 24303851|
|Oral Dis.||Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome Pubmed:24106991|
|Endocrine||Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia Pubmed:24840719|
|The Clinical Respiratory Journal||Relationship between plasma chemerin levels and disease severity in COPD patients Pubmed:24865134|
|Gynecol Endocrinol.||Elevated serum chemerin in Chinese women with hyperandrogenic PCOS Pubmed:24927079|
|Endocrine.||Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. Pubmed:25471281|
|Obesity Surgery||Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126|
|Dermatologica Sinica||Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris Sciencedirect:S1027811714000470|
|Dis Markers||Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study PubMed: 26170530|
|Med Sci Monit||Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population PubMed: 26471865|
|Endocrine||Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats PubMed: 25471281|
|Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids||Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes Pubmed:26721419|
|Medicine (Baltimore).||Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen. pubmed:27583869|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA945Mu01||Recombinant Chemerin (CHEM)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA945Mu02||Recombinant Chemerin (CHEM)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA945Mu02||Polyclonal Antibody to Chemerin (CHEM)||WB; IHC; ICC; IP.|
|PAA945Mu01||Polyclonal Antibody to Chemerin (CHEM)||WB; IHC; ICC; IP.|
|SEA945Mu||ELISA Kit for Chemerin (CHEM)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSA945Mu01||ELISA Kit DIY Materials for Chemerin (CHEM)||Main materials for "Do It (ELISA Kit) Yourself".|