ELISA Kit for Serum Amyloid P Component (SAP)
APCS; PTX2; Pentaxin-Related; 9.5S Alpha-1-Glycoprotein
- Product No.SEB539Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.37ng/mL.
- DownloadInstruction Manual
96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 448
For more details, please contact local distributors! US$ 2016
For more details, please contact local distributors! US$ 3808
For more details, please contact local distributors! US$ 31360
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Serum Amyloid P Component (SAP).
No significant cross-reactivity or interference between Serum Amyloid P Component (SAP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Serum Amyloid P Component (SAP) and the recovery rates were calculated by comparing the measured value to the expected amount of Serum Amyloid P Component (SAP) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Serum Amyloid P Component (SAP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Serum Amyloid P Component (SAP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Journal of Vascular Surgery||Circulation levels of acute phase proteins in patients with Takayasu arteritis PubMed: 20100644|
|Journal of Proteome Research||Combined Proteomic and Metabolomic Profiling of Serum Reveals Association of the Complement System with Obesity and Identifies Novel Markers of Body Fat Mass Changes ACS: pr2005555|
|Liver International||Identification of candidate biomarkers for hepatocellular carcinoma in plasma of HCV-infected cirrhotic patients by 3-D DIGE Pubmed: 23944848|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPB539Mu01||Recombinant Serum Amyloid P Component (SAP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB539Mu02||Recombinant Serum Amyloid P Component (SAP)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB539Mu01||Polyclonal Antibody to Serum Amyloid P Component (SAP)||WB; IHC; ICC; IP.|
|PAB539Mu02||Polyclonal Antibody to Serum Amyloid P Component (SAP)||WB; IHC; ICC; IP.|
|SEB539Mu||ELISA Kit for Serum Amyloid P Component (SAP)||Enzyme-linked immunosorbent assay for Antigen Detection.|