ELISA Kit for Jagged 1 (JAG1)

CD339; AGS; AHD; AWS; HJ1; JAGL1; Alagille Syndrome; Jagged 1 Protein

Specificity

This assay has high sensitivity and excellent specificity for detection of Jagged 1 (JAG1).
No significant cross-reactivity or interference between Jagged 1 (JAG1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Jagged 1 (JAG1) and the recovery rates were calculated by comparing the measured value to the expected amount of Jagged 1 (JAG1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-103 93
EDTA plasma(n=5) 96-104 99
heparin plasma(n=5) 87-98 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Jagged 1 (JAG1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Jagged 1 (JAG1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Jagged 1 (JAG1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 81-95% 87-98% 80-92%
EDTA plasma(n=5) 97-104% 84-98% 85-98% 91-105%
heparin plasma(n=5) 92-103% 91-102% 96-104% 78-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
13 Cross-talk between the Toll-like receptor 4 and Notch1 pathways augments the inflammatory response in the interstitial cells of stenotic human aortic valves PubMed: PMC3506187
Eur J Obstet Gynecol Reprod Biol. Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis Pubmed: 24188612
PLoS One. Augmented Osteogenic Responses in Human Aortic Valve Cells Exposed to oxLDL and TLR4 Agonist: A Mechanistic Role of Notch1 and NF-κB Interaction Pubmed:Pmc4014478
Biochim Biophys Acta.? Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expression Pubmed:25101972
international journal of oral-medical sciences Jagged1 Stimulates Bone Resorption during Orthodontic Tooth Movement Dn: Jlc
Oncology letters MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer
Journal of Biological Chemistry Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328
Cell Death & Disease Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria 33795655
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB807Hu04 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB807Hu02 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB807Hu03 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB807Hu01 Recombinant Jagged 1 (JAG1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB807Hu01 Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC; IP.
PAB807Hu03 Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC; IP.
PAB807Hu02 Polyclonal Antibody to Jagged 1 (JAG1) WB
LAB807Hu71 Biotin-Linked Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC.
LAB807Hu73 Biotin-Linked Polyclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC.
MAB807Hu22 Monoclonal Antibody to Jagged 1 (JAG1) WB; IHC; ICC; IP.
MAB807Hu21 Monoclonal Antibody to Jagged 1 (JAG1) WB
SEB807Hu ELISA Kit for Jagged 1 (JAG1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB807Hu Multiplex Assay Kit for Jagged 1 (JAG1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB807Hu01 ELISA Kit DIY Materials for Jagged 1 (JAG1) Main materials for "Do It(ELISA Kit) Yourself"