• Star-Product
ELISA Kit for Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1)

OLR1; CLEC8A; SCARE1; Oxidized low-density lipoprotein receptor 1; C-type lectin domain family 8 member A; Lectin-like oxidized LDL receptor 1; Lectin-type oxidized LDL receptor 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1).
No significant cross-reactivity or interference between Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) and the recovery rates were calculated by comparing the measured value to the expected amount of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-104 99
EDTA plasma(n=5) 79-101 97
heparin plasma(n=5) 82-102 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-104% 98-105% 94-101% 98-105%
EDTA plasma(n=5) 92-101% 84-96% 91-99% 80-102%
heparin plasma(n=5) 79-103% 78-104% 83-92% 79-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Clinical Cardiology Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels Are Associated With Angiographic Coronary Lesion Complexity in Patients With Coronary Artery Diseas PubMed: 21400544
Coronary Artery Disease Postprocedural serum sLOX-1 levels are associated with coronary in-stent restenosis in patients with stable coronary artery disease LWW: 06000
Clinical Biochemistry Soluble lectin-like oxidized low-density lipoprotein receptor-1 levels in synovial fluid are correlated with disease severity of knee osteoarthritis ScienceDirect: S0009912011004905
Mediators of Inflammation Pregnancy Followed by Delivery May Affect Circulating Soluble Lectin-Like Oxidized Low-Density Lipoprotein Receptor-1 Levels in Women of Reproductive Age PubMed: 22619487
Dicle Medical Journal Lectin-like oxidized low density lipoprotein receptor 1(LOX-1) levels and endothelial dysfunction in patients with primary essential hyperhidrosis/Primer esansiyel hiperhidroz hastalarinda lektin benzeri okside düsük dansiteli lipoprotein reseptor 1(LOX-1) düzeyleri ve endotel disfonksiyonu Readperiodicals: Source
Journal of Clinical Laboratory Analysis Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome Pubmed:24798146
Journal of Human Hypertension Endothelial damage in white coat hypertension: role of lectin-like oxidized low-density lipoprotein-1 Pubmed:25007999
Gynecologic and Obstetric Investigation Inhibition of Lectin-Like Oxidized Low-Density Lipoprotein Receptor 1 Protects against Plasma/Hypoxia-Mediated Trophoblast Dysfunction Associated with Preeclampsia Pubmed:25633310
World J Gastroenterol Increased serum soluble lectin-like oxidized low-density lipoprotein receptor-1 levels in patients with biopsy-proven nonalcoholic fatty liver disease PubMed: 26185381
Genetic Testing and Molecular Biomarkers Serum sLOX-1 Levels Are Correlated with the Presence and Severity of Obstructive Sleep Apnea PubMed: 25825846
Neurotoxicology. 27-Hydroxycholesterol regulates cholesterol synthesis and transport in C6 glioma cells. pubmed:28167099
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB859Mu01 Recombinant Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) Positive Control; Immunogen; SDS-PAGE; WB.
RPB859Mu02 Recombinant Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB859Mu02 Polyclonal Antibody to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) WB; IHC; ICC; IP.
PAB859Mu01 Polyclonal Antibody to Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) WB; IHC; ICC; IP.
SEB859Mu ELISA Kit for Lectin Like Oxidized Low Density Lipoprotein Receptor 1 (LOX1) Enzyme-linked immunosorbent assay for Antigen Detection.