ELISA Kit for Fatty Acid Synthase (FASN)

FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X,Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase

Specificity

This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN).
No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121
Oncogenesis. Diet-induced alteration of fatty acid synthase in prostate cancer progression Pubmed:26878389
Animal Science Journal Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells pubmed:28804941
life sciences Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma Pubmed: 30914315
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
bmc research notes The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC470Mu02 Recombinant Fatty Acid Synthase (FASN) Positive Control; Immunogen; SDS-PAGE; WB.
RPC470Mu01 Recombinant Fatty Acid Synthase (FASN) Positive Control; Immunogen; SDS-PAGE; WB.
PAC470Mu02 Polyclonal Antibody to Fatty Acid Synthase (FASN) WB; IHC; ICC; IP.
PAC470Mu01 Polyclonal Antibody to Fatty Acid Synthase (FASN) WB; IHC; ICC; IP.
SEC470Mu ELISA Kit for Fatty Acid Synthase (FASN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC470Mu Multiplex Assay Kit for Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.