ELISA Kit for Glutathione Peroxidase 3, Plasma (GPX3)
GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase
- Product No.SEC992Hu
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Sample TypeSerum, plasma, breast milk and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range1.56-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.62ng/mL.
- DownloadInstruction Manual
Ensure in stock; if not, free redo!
- FOB US$ 490
For negotiated price and more details, please contact local distributors! US$ 700
For negotiated price and more details, please contact local distributors! US$ 3150
For negotiated price and more details, please contact local distributors! US$ 5950
For negotiated price and more details, please contact local distributors! US$ 49000
For negotiated price and more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Glutathione Peroxidase 3, Plasma (GPX3).
No significant cross-reactivity or interference between Glutathione Peroxidase 3, Plasma (GPX3) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Glutathione Peroxidase 3, Plasma (GPX3) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione Peroxidase 3, Plasma (GPX3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Peroxidase 3, Plasma (GPX3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Peroxidase 3, Plasma (GPX3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione Peroxidase 3, Plasma (GPX3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
|Reproduction||Alteration in the intrafollicular thiol–redox system in infertile women with endometriosisPubmed:25376627|
|international journal of cardiology||Endothelial function impairment in STEMI patients with out-of-hospital cardiac arrest under therapeutic hypothermia treatment.pubmed:28087172|
|Current Alzheimer Research||Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients.pubmed:27748187|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications|
|RPC992Hu01||Recombinant Glutathione Peroxidase 3, Plasma (GPX3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC992Hu01||Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)||WB; IHC; ICC; IP.|
|MAC992Hu22||Monoclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)||WB; IHC; ICC; IP.|
|SEC992Hu||ELISA Kit for Glutathione Peroxidase 3, Plasma (GPX3)||Enzyme-linked immunosorbent assay for Antigen Detection.|