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Good news, primary cardiac myocytes are online
Currently, Cloud-Clone Corp. can provide large numbers of neonatal rat myocardial cells (CSI007Ra01). Primary cardiac myocytes are oval or irregular star, and rhythmically beating. And they can be supplied as the fresh or frozen form with high purity and survival, so they could be used in the cardiovascular, cardiac tissue research and a variety of physiological and biochemical experiments.As we know, cell culture in vitro can supplement the shortage of in vivo test, and it can make the cell function research more operable. However, it is more and more difficult to be convinced of the cell line data. Cell line misidentification and impurity, different genetic phenotype and other factor......
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Cytokines and health in higher animals
Cytokines are a kind of low molecular weight soluble proteins which are formed in process of evolution for higher animals. They have many functions, such as regulation of innate immunity, adaptive immunity, blood cell formation, cell growth, tissue repair and so on. The types of cytokines which have been found recently are more than 100. According to the different functions, they can be divided into interleukin (IL), interferon (IFN), colony stimulating factor (CSF), tumor necrosis factor (TNF), erythropoietin (EPO) and other types. With the in-depth study, the total number of cytokines are changing and increasing year by year.Cytokines are produced by specific cells and secreted into ......
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The battle of PD-1/PD-L1 and cancer cells
PD-1, also known as programmed cell death protein 1, is a protein that is encoded by the PDCD1 gene. PD-1 has two companions: one is known as PD-L1, the other is PD-L2. Both of them are the ligands of the PD-1. PD-1 is mainly expressed in immune cells, PD-L1 is expressed in cancer cells, and PD-L2 is mainly expressed in the dendritic cells. Compared to the PD-L1, the expression range of PD-L2 is relatively narrow, and the research of PD-L2 is less. So we mainly introduce the PD-1 and PD-L1 this time.First of all, let us look at the relationship between cancer cells and PD-1/PD-L1.The shut down of T-cell-mediated immune response is one of the ways that cancer cells evaded from body’s ......
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Lentivirus and adenovirus expression system
Virus, as a vector for exogenous gene espression, has been widely applied in many fields, including fucntional verification of gene, small RNA, transcription factor, recombinant protein production and transgenic animal research, et al. With the characteristic of virus infection and recombinant plasmid which contains viral genome, exogenous gene and others, protein overexpression or interference could be performed in mammalian cells.The technical system is mainly composed of two parts: virus packaging and virus infection (Fig1 and Fig 2). The virus packaging is the process of preparation recombinant plasmids by genetic engineering method. It can be divided into lentiviral packaging syst......
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How to use the antibody pairs?
For immunoassay kit manufacturers, diagnostic reagent manufacturers, pharmaceutical factories which have large demands for detection reagents, the biggest obsession is the limited availability of raw materials. This knotty problem is smoothly solved as the antibody pairs were developed by Cloud-Clone Corp.. With high-quality antibody pairs, the efficient immunoassay detection assays can be developed, including ELISA, CLIA, ELISPOT, Luminex, Immunochromatography and other immunoassays.Here the reagents composition for antibody pairs are introduced in detail, shown in table 1. In addition, two common usages-sandwich ELISA and immunochromatography (colloid gold-immunochromatography assa......
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A brief introduction of gene editing techniques CRISPR/Cas9 and Argonaute
The development of science and technology makes people to know the disease deeply. It was found that the etiology of many human diseases has an inseparable relationship with gene mutation. So, how to treat these diseases? People naturally think of re-editing mutant gene by using some techniques, this technology is gene editing technique. Based on protein/DNA identification and cut, gene editing techniques have been through the first generation of gene editing techniques, such as homing endonuclease, ZFN and TALEN. With the constantly understanding of the gene editing techniques, people realized the first generation of gene editing techniques have many defects, such as fussy operation, ......