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Cloud-Clone in vitro diagnostic reagent raw material
In vitro diagnosis(IVD) refers to the products and services that outside the human body, through detecting the human body samples (blood, body fluids, tissues, etc.) to obtain clinical diagnostic information, and then judge the disease or the body function. With the continuous development of science and technology, the in vitro diagnostic technique tends to be more and more simple, diversified, high throughput and precision. The development of in vitro diagnostic techniques is inseparable from the innovation of diagnostic reagents, which have gone through four technological revolution as chemical, enzyme, immunoassay and probe technology. Among them, the development of the immune detec......
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The detection of bone resorption marker CTXI
CTXI is type I collagen cross-linking carboxyterminal telopeptide, type I collagen consists of three peptide chains, two of them are α(I) chains, the rest is α(Ⅱ) chain, three uncoiling short peptides exist in the carboxyl terminal of mature type I collagen. CTXI is a telopeptide which is disaggregated from the terminal of type I collagen. Due to the collagen with unique triplex super helical structure, its property is very stable, it cannot be resolved by the general processing temperature and short time heating, it is hard to be digestive absorption and not easy for the body to make full use of.Type I collagen mainly distributes in the skin, bone, cornea, muscle tendon and tumour......
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Structure modification of small molecule haptens
To be prepared the antibodies against small molecules, the structure of small molecules is important, and it is commonly to have a certain complexity and rigidity, including benzene rings, heterocyclic groups or branched structures, otherwise, it would be difficult to produce antibodies with good titers.Some haptens have a reactive group, such as -COOH, -NH2, -OH, etc., and these haptens could be directly conjugated to a carrier protein, and then to be a totally artificial antigen, please see the the principle of coupling in Fig1.. However, the haptens should be re-designed and modified if there are no reactive groups which could be covalently conjugated to a carrier, or though the hap......
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E.coli cell free expression system
The traditional E. coli expression system has been continuously improved and optimized. It has the advantages of short production cycle, high efficiency, low cost and so on. But it also has the defects of the system itself. E. coli expression system can not complete the folding and modification of eukaryotic proteins. So far, this system still can not be used for expressing the membrane protein and some toxic protein expression, which seriously restricts the application of E.coli Expression System. In order to make up for the defects of the prokaryotic expression system, the eukaryotic expression system is commonly used to express the recombinant protein.But the eukaryotic expression s......
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The precision analysis of Enzyme-linked immunosorbent assay kits
Precision means the testing consistency of measured values obtained by repeated test with same sample under same condition. As one of the basic analytical performance of the detection system, it is the basis of validity assessment for detection system. Well, how to measure the precision of ELISA kit?The precision of ELISA kit is divided into ‘Intra-assay Precision’ and ‘Inter-assay Precision’. It is shown in coefficient of variation(CV).Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level analytes were tested 20 times on one plate, respectively. Then the Mean and standard deviation(SD) were calculated by following formulas.Inter-assay Pre......
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Assay kits for common tumor markers from Cloud-Clone Corp.
Tumor is a neoplasm which forms on the local tissue, due to the stimulation of various tumorigenic factors. It can be divided into benign and malignant tumor according to the biological characteristics of tumor cell and the damage degree to the body. Malignant tumor is also know as cancer, which is one of the main causes of death in the world nowadays. It has caused a serious hazard to human-beings, and restrict the social and economic development. Because of this, study of cancers has always been a hot spot in the field of biomedical research.Tumor markers refer to substances produced in malignant cells or host cells which is generated by the stimulation of malignant cells. It can ref......