First Edition (Revised on April, 2016)
PBS and TRIS buffer is widely used in biological experiments, such as Immunohistochemistry (IHC), Western Blot (WB), Immunofluorescence (IF), enzyme linked immunosorbent assays (ELISAs), etc.
|IS045-1||PBS Buffer (powder) (0.01M, pH7.2-7.6)||1 bag / 1 box; 12 bags / 1 box|
|IS045-2||TRIS Buffer (powder) (0.02M, pH8)||1 bag / 1 box; 12 bags / 1 box|
STORAGE AND PERIOD OF VALIDITY
Store at a cool place. The Period of Validity is 1 year.
Power in bags, 12 bags in one box.
Dissolve one bag of buffer in 1L of distilled water, mix, adjust pH to 8.0 with HCl and store the buffer solution at 4℃. Sterilization can be performed by filtration. Filter the buffer solution through a 0.22 μm filter into a sterile flask or autoclave the solution for 15 to 20 minutes.
Please avoid microbial contamination while using the buffer.
Precipitates may appear at low temperatures. If it happens, put the bottle in water bath at 37℃. For your safety and health, please wear lab coat and disposable gloves in experiment.