Staining Solution for Cells and Tissue

Instruction manual


First Edition (Revised on April, 2016)


The staining technology is one of the important methods to study the pathology. Pathology teaching, scientific research, clinical biopsy and diagnosis can not do without pathological staining technology. In order to display and determine the tissues or cells in the normal structure or in the pathological process of abnormal substances, diseases and pathogens. Appropriate staining methods are for different tissues and different purposes.

Products and application

Product NameApplicationSection typeFormat
Hematoxylin eosin (HE) staining solutionHematoxylin stains the nucleus chromatin and cytoplasm ribosome with purple blue, eosin stains the cytoplasm and the extracellular matrix components with red.Paraffin section Frozen section CellsHematoxylin solution: 100ML
Eosin Solution: 100ML
Masson staining solutionMasson staining was mainly used for the differential staining of collagen fibers and muscle fibers. Collagen and cartilage was blue, muscle fiber, cellulose and red blood cell stained red, nuclei stained blue black.Paraffin section Frozen sectionWeigert's iron hematoxylin A: 20ML
Weigert's iron hematoxylin B: 20ML
Ponceau red Fuchsine Acid: 20ML
1% phosphomolybdic acid: 20ML
               2% acetic acid aniline blue: 20ML
Alcian blue and periodic acidAB-PAS staining is used to classify the gastric mucosalParaffin sectionAlcian blue solution: 20ML
Schiff (AB-PAS) staining solutionepithelial metaplasia of gastric mucosa, and to observe the changes of the morphology of glycogen and mucous substance. Acid mucus was blue, neutral mucus was red, mixed mucus was purple, the nucleus was pale blueFrozen sectionSchiff reagent: 20ML
Periodic acid: 20ML
Mayer hematoxylin: 20ML
Sulfinic acid wash buffer: 100ML
Alcian blue staining solutionAlcian blue belongs to cationic dyes, is the most specific dye for acid mucus.Paraffin section Frozen sectionAlcian blue(PH=2.5) solution: 100ML
Nuclear Fast Red solution: 100ML
Triphenyl tetrazolium chloride (TTC) staining solutionTriphenyl tetrazolium chloride (TTC) is a fat soluble light sensitive compound, and normal tissue in the dehydrogenase reaction were red, and tissue ischemia is pale.Fresh tissue(such as brain tissue)TTC solution: 100ML
Periodate Schiff (PAS) staining solutionPAS staining can show the polysaccharide, neutral mucus and some acidic mucus substances, as well as cartilage, pituitary, fungi, basement membrane and other substances.Paraffin section Frozen section CellsSchiff reagent: 20ML
Periodate: 20ML
Mayer hematoxylin: 20ML
Sulfinic acid wash solution: 100ML
Van Gieson (VG) staining solutionVan Gieson (VG) staining is used to distinguish between collagen fibers and muscle fibers. Staining solution is picric acid and acid fuchsin mixture, the collagen fibers were acid fuchsin stained pink or red, muscle fibers were dyed yellow picric acidParaffin section Frozen sectionan Gieson A: 5ML
Van Gieson B: 60ML
Weigert’s iron hematoxylin A: 20ML
               Weigert’s iron hematoxylin B: 20ML
Oil red O staining solutionOil red O belongs to azo dyes, it is a strong fat solvent and dye, The fat droplets in the tissue are orange.Frozen section CellsOil red O solution: 100ML
Annona Red-Fast Green staining solutionAnnona Red-Fast Green staining  is the common staining of articular cartilage, cartilage and bone tissue.Paraffin section Frozen sectionAnnona Red: 100ML
Fast Green: 100ML
1% phosphomolybdic acid: 100ML

Storage and Validity

Different components are stored in the 4°C, -20°C respectively, valid for one year.

Important Note

1.Reagents should be stored according to the instructions.
2.Paraffin section/slice need complete dewaxing.
3.The time of periodate oxidation of tissue sections is within 10 minutes, the ambient temperature is not more than 20°C.