Flow Cytometry (FCM) Experiment Service
First Edition (Revised on April, 2016)
Flow cytometry (FCM) is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. FCM can not only measure the cell size, internal particles of traits, but also can detect cell surface and cytoplasmic antigen, intracellular DNA, RNA content. FCM have been widely used in hematology, immunology, oncology, pharmacology and molecular biology.
Immune Cell Typing
Surface Antigen Identification
|Service Items||Sample preparation||Reagents|
|Cell Sorting Service||Whole Blood||Specific antibody (provided by customer)|
|Cell Apoptosis Service||Freshly cultured cells||Annexin V-FITC cell apoptosis detection kit|
|Cell Cycle Service||Trypsin digestion and make a single cell suspension||RNA enzyme, PI dye, etc.|
|Immunocytes Typing Service||Cultured cells, stimulated cells, anticoagulant whole blood, tissue homogenate||Membrane breaking agent, fixing agent, stimulating agent, specific antibody (provided by customer.|
|Surface Antigen Identification||Cultured cells||Specific antibody ( provided by customer)|
Single cell: 1~2 * 106 cells;
Peripheral blood: EDTA or heparin anticoagulant whole blood, 5ml;
Species: human, mouse, rat or rabbit and other species.
Common used fluorescent probes, such as PI (free of charge); special markers, such as CD antibody, the customers should provide them, and our company can purchase them for customers; Antibody should be able to identify the specific antigen on samples.
Samples，such as whole blood, cells etc.
Please provide sample information and processing method, etc.
Provides complete experimental protocol, data and images.