Assay Kit DIY Support Pack 2

Instruction manual

FOR IN VITRO AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

First Edition (Revised on April, 2016)

PRODUCT INFORMATION

The Assay Kit DIY Support Pack is intended to use together with Assay Kit DIY Materials for ELISA kit (competitive method) preparation. This pack provides sufficient materials and reagents for preparation of 10 Competitive ELISA/CLIA plates. All reagents should be used at room temperature and the pack is intended for research purposes only.

REAGENTS AND MATERIALS PROVIDED

ReagentsQuantity
Plate Sealer40 pieces
Coating Buffer1 bottles of powder
Blocking Buffer1 bottles of powder
Reagent Diluent 11 bottles of powder
Reagent Diluent 21 bottles of powder
Reagent Diluent 31 bottles of powder
Wash Buffer2 bottles of powder
Enhancer1 tube of liquid

Applications

ReagentsApplications
Plate SealerUse to cover the wells while incubation.
Coating BufferUsed for coating antibody on the wells of 96-well strip plate. Prepare a working solution by diluting the Coating Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 220ml working solution.
Blocking BufferUsed for blocking of unspecific binding sites of the plate. Prepare a working solution by diluting the Blocking Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 240ml working solution.
Reagent Diluent 1Used for dilution of Standard. Prepare a working solution by diluting the reagent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 160ml working solution.
Reagent Diluent 2Used for dilution of Biontin-labeled Competitor. Prepare a working solution by diluting the reagent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 120ml working solution.
Reagent Diluent 3Used for dilution of Streptavidin-HRP. Prepare a working solution by diluting the reagent with deionized or distilled water before the experiment. 1 bottle can be used for preparing 120ml working solution.
Wash BufferFor plate washing. Prepare a working solution by diluting the Wash Buffer with deionized or distilled water before the experiment. 1 bottle can be used for preparing 3200ml working solution.
EnhancerUsed to enhance the effect of blocking. Please add 50ul Enhancer into 100ml the Blocking Buffer working solution.

NOTE: *All of the working solutions should be clear.
*If working solution is prepared, please store at 2-8ºC, and use up within one week.

Storage

This pack is valid for one year at room temperature. Please make all solutions fresh before the experiment.

IMPORTANT NOTES

Appropriate care should be exercised when using these reagents, including gloves, glasses, lab coats, and good laboratory procedures.