Streptavidin-HRP

Instruction manual

First Edition (Revised on April, 2016)

[PRODUCT INFORMATION]

HRP-labeled Streptavidin is named HRP-Streptavidin or Streptavidin-HRP. It is recommended for use in detection systems utilizing biotinylated antibodies, and other biotinylated molecules. This product is documented for use in a variety of common applications including Immunohistochemistry, Western Blot, In Situ Hybridization, and ELISA by catalyzing ECL reagent (such as ECL, BeyoECL, etc.). And It can catalyze TMB to become blue at ELISA and DAB to produce brown precipitation at Immunohistochemistry, Immunocytochemistry, or Western Blot.

[PRODUCT LIST]

Component

IS089-1

IS089-2

IS089-3

Streptavidin-HRP

0.2ml

0.6ml

1.2ml

Instruction Manual

1

[USAGE]

This product can be used in Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), In Situ Hybridization (ISH), etc. We recommend the following ranges as starting points.

WB

IHC/ICC/ISH

ELISA

Southern/Northern

EMSA

1:2000-10000

1:200-500

1:2000-10000

1:2000-5000

1:2000-5000

[STORAGE AND PERIOD OF VALIDITY]

Store at -20℃. The Period of validity is 1 year.

[IMPORTANT NOTES]

1. Sodium azide will inhibit the activity of horseradish peroxidase(HRP), so don’t add sodium azide into solutions.

2. This product is limited to the professional scientific research and not for use in clinical diagnosis.

3. For your safe and healthy, please wear lab coat and disposable gloves.