Tissue Plasminogen Activator (tPA)

[Edit]

PLAT; T-PA; t-plasminogen activator; Alteplase; Reteplase

Tissue Plasminogen Activator (tPA)

Tissue plasminogen activator is a protein involved in the breakdown of blood clots. Specifically, it is a serine protease (EC 3.4.21.68) found on endothelial cells, the cells that line the blood vessels. As an enzyme, it catalyzes the conversion of plasminogen to plasmin, the major enzyme responsible for clot breakdown. Because it works on the clotting system, tPA is used in clinical medicine to treat only embolic or thrombolytic stroke. Use is contraindicated in hemorrhagic stroke and head trauma.The classic role of tPA is in the clotting system. Specifically, tPA catalyzes the conversion of plasminogen into plasmin. It does so by cleaving the single-chained plasminogen into two chains. These two chains are linked by a disulfide bond and the resulting molecule is called plasmin.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA525Hu01 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu02 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu04 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu05 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Hu03 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
EPA525Hu62 Eukaryotic Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
EPA525Hu61 Eukaryotic Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA525Hu01 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
MAA525Hu22 Monoclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
PAA525Hu02 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
MAA525Hu26 Monoclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
MAA525Hu23 Monoclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
Assay Kits SEA525Hu ELISA Kit for Tissue Plasminogen Activator (tPA) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA525Mu01 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
RPA525Mu02 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
APA525Mu01 Active Tissue Plasminogen Activator (tPA) Cell culture; Activity Assays.
Antibodies PAA525Mu01 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
LAA525Mu71 Biotin-Linked Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC.
PAA525Mu02 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
Assay Kits SEA525Mu ELISA Kit for Tissue Plasminogen Activator (tPA) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA525Ra01 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA525Ra01 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
MAA525Ra21 Monoclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
Assay Kits SEA525Ra ELISA Kit for Tissue Plasminogen Activator (tPA) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA525Po01 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA525Po01 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
MAA525Po21 Monoclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
Assay Kits SEA525Po ELISA Kit for Tissue Plasminogen Activator (tPA) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli."Nature 301:214-221(1983) [PubMed] [Europe PMC] [Abstract]
  2. "The structure of the human tissue-type plasminogen activator gene: correlation of intron and exon structures to functional and structural domains."Proc. Natl. Acad. Sci. U.S.A. 81:5355-5359(1984) [PubMed] [Europe PMC] [Abstract]
  3. "The human tissue plasminogen activator gene."J. Biol. Chem. 261:6972-6985(1986) [PubMed] [Europe PMC] [Abstract]
  4. "Cloning of cDNA coding for human tissue-type plasminogen activator and its expression in Escherichia coli."Mol. Biol. Med. 3:279-292(1986) [PubMed] [Europe PMC] [Abstract]
  5. "Expression of human uterine tissue-type plasminogen activator in mouse cells using BPV vectors."DNA 6:461-472(1987) [PubMed] [Europe PMC] [Abstract]
  6. "Nucleotide sequence of the tissue-type plasminogen activator cDNA from human fetal lung cells."Nucleic Acids Res. 16:5695-5695(1988) [PubMed] [Europe PMC] [Abstract]
  7. "Variant tissue-type plasminogen activator (PLAT) cDNA obtained from human endothelial cells."Nucleic Acids Res. 18:1086-1086(1990) [PubMed] [Europe PMC] [Abstract]
  8. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  10. "Isolation and characterization of the human tissue-type plasminogen activator structural gene including its 5' flanking region."J. Biol. Chem. 260:11223-11230(1985) [PubMed] [Europe PMC] [Abstract]
  11. "Purification and characterization of tissue plasminogen activator secreted by human embryonic lung diploid fibroblasts, IMR-90 cells."Agric. Biol. Chem. 55:1225-1232(1991) [PubMed] [Europe PMC] [Abstract]
  12. "Purification and characterization of a melanoma cell plasminogen activator."Eur. J. Biochem. 132:681-686(1983) [PubMed] [Europe PMC] [Abstract]
  13. "Tissue plasminogen activator: peptide analyses confirm an indirectly derived amino acid sequence, identify the active site serine residue, establish glycosylation sites, and localize variant differences."Biochemistry 23:3701-3707(1984) [PubMed] [Europe PMC] [Abstract]
  14. "Isolation of cDNA sequences coding for a part of human tissue plasminogen activator."Proc. Natl. Acad. Sci. U.S.A. 80:349-352(1983) [PubMed] [Europe PMC] [Abstract]