Nucleoporin 98 (NUP98)

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is composed of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromsome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL).

Organism species: Homo sapiens (Human)

Proteins RPB196Hu01 Recombinant Nucleoporin 98 (NUP98) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB196Hu01 Polyclonal Antibody to Nucleoporin 98 (NUP98) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Nucleoporin 98 (NUP98) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nucleoporin 98 (NUP98) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

Proteins RPB196Mu01 Recombinant Nucleoporin 98 (NUP98) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB196Mu01 Polyclonal Antibody to Nucleoporin 98 (NUP98) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Nucleoporin 98 (NUP98) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nucleoporin 98 (NUP98) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

Proteins n/a Recombinant Nucleoporin 98 (NUP98) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Nucleoporin 98 (NUP98) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Nucleoporin 98 (NUP98) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Nucleoporin 98 (NUP98) CLIA Kit Customized Service Offer
n/a ELISA Kit for Nucleoporin 98 (NUP98) ELISA Kit Customized Service Offer
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  2. "A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96."J. Cell Biol. 144:1097-1112(1999) [PubMed] [Europe PMC] [Abstract]
  3. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "The full-ORF clone resource of the German cDNA consortium."BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
  6. "The t(11;20)(p15;q11) chromosomal translocation associated with therapy-related myelodysplastic syndrome results in an NUP98-TOP1 fusion."Blood 94:3258-3261(1999) [PubMed] [Europe PMC] [Abstract]
  7. "RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains."J. Cell Biol. 145:237-254(1999) [PubMed] [Europe PMC] [Abstract]
  8. "Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98."Mol. Cell 6:1243-1252(2000) [PubMed] [Europe PMC] [Abstract]
  9. "A novel gene, NSD1, is fused to NUP98 in the t(5;11)(q35;p15.5) in de novo childhood acute myeloid leukemia."Blood 98:1264-1267(2001) [PubMed] [Europe PMC] [Abstract]
  10. "Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export."J. Cell Biol. 155:339-354(2001) [PubMed] [Europe PMC] [Abstract]
  11. "NUP98 is fused to the NSD3 gene in acute myeloid leukemia associated with t(8;11)(p11.2;p15)."Blood 99:3857-3860(2002) [PubMed] [Europe PMC] [Abstract]
  12. "Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export."J. Cell Biol. 156:617-630(2002) [PubMed] [Europe PMC] [Abstract]
  13. "Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex."Mol. Biol. Cell 14:1923-1940(2003) [PubMed] [Europe PMC] [Abstract]
  14. "Nucleoporins as components of the nuclear pore complex core structure and Tpr as the architectural element of the nuclear basket."Mol. Biol. Cell 15:4261-4277(2004) [PubMed] [Europe PMC] [Abstract]
  15. "Characterization of 6q abnormalities in childhood acute myeloid leukemia and identification of a novel t(6;11)(q24.1;p15.5) resulting in a NUP98-C6orf80 fusion in a case of acute megakaryoblastic leukemia."Genes Chromosomes Cancer 44:225-232(2005) [PubMed] [Europe PMC] [Abstract]
  16. "t(9;11)(p22;p15) with NUP98-LEDGF fusion gene in pediatric acute myeloid leukemia."Leuk. Res. 29:467-470(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
  18. "NUP98 rearrangements in hematopoietic malignancies: a study of the Groupe Francophone de Cytogenetique Hematologique." Leukemia 20:696-706(2006) [PubMed] [Europe PMC] [Abstract]
  19. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  20. "A novel NUP98-PHF23 fusion resulting from a cryptic translocation t(11;17)(p15;p13) in acute myeloid leukemia."Leukemia 21:842-844(2007) [PubMed] [Europe PMC] [Abstract]
  21. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
  22. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
  23. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  24. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  25. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  26. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  28. "Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion."EMBO J. 29:1659-1673(2010) [PubMed] [Europe PMC] [Abstract]
  29. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  31. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
  32. "Functional analysis of the NUP98-CCDC28A fusion protein."Haematologica 97:379-387(2012) [PubMed] [Europe PMC] [Abstract]
  33. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  34. "The three-dimensional structure of the autoproteolytic, nuclear pore-targeting domain of the human nucleoporin Nup98."Mol. Cell 10:347-358(2002) [PubMed] [Europe PMC] [Abstract]
  35. "Structural constraints on autoprocessing of the human nucleoporin Nup98."Protein Sci. 17:494-505(2008) [PubMed] [Europe PMC] [Abstract]
  36. "Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1."Proc. Natl. Acad. Sci. U.S.A. 107:10406-10411(2010) [PubMed] [Europe PMC] [Abstract]
  37. "The consensus coding sequences of human breast and colorectal cancers." Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract]