Ninein (NIN)

[Edit]

GSK3B Interacting Protein; Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein

Ninein (NIN)
Ninein is one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.The deduced 2,047-amino acid protein has a calculated molecular mass of 239 kD. NIN contains an N-terminal GTP-binding site, a large coiled-coil domain with 4 leucine zipper motifs, and a C-terminal domain responsible for GSK binding. Unlike mouse Nin, it does not have an N-terminal Ca(2+)-binding site. Northern blot analysis detected a 9.0-kb transcript in all tissues examined and a 7.0-kb transcript predominantly in heart and skeletal muscle.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC657Hu01 Recombinant Ninein (NIN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC657Hu01 Polyclonal Antibody to Ninein (NIN) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Ninein (NIN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ninein (NIN) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC657Mu01 Recombinant Ninein (NIN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC657Mu01 Polyclonal Antibody to Ninein (NIN) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Ninein (NIN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ninein (NIN) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPC657Ra01 Recombinant Ninein (NIN) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAC657Ra01 Polyclonal Antibody to Ninein (NIN) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for Ninein (NIN) CLIA Kit Customized Service Offer
n/a ELISA Kit for Ninein (NIN) ELISA Kit Customized Service Offer
  1. "Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta."Biochim. Biophys. Acta 1492:513-516(2000) [PubMed] [Europe PMC] [Abstract]
  2. "Genomic organization and molecular characterization of the human ninein gene."Biochem. Biophys. Res. Commun. 279:989-995(2000) [PubMed] [Europe PMC] [Abstract]
  3. "Human ninein is a centrosomal autoantigen recognized by CREST patient sera and plays a regulatory role in microtubule nucleation."Cell Cycle 3:923-930(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."DNA Res. 7:273-281(2000) [PubMed] [Europe PMC] [Abstract]
  5. "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones."DNA Res. 9:99-106(2002) [PubMed] [Europe PMC] [Abstract]
  6. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  8. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  9. "CEP110 and ninein are located in a specific domain of the centrosome associated with centrosome maturation."J. Cell Sci. 115:1825-1835(2002) [PubMed] [Europe PMC] [Abstract]
  10. "Assembly of centrosomal proteins and microtubule organization depends on PCM-1."J. Cell Biol. 159:255-266(2002) [PubMed] [Europe PMC] [Abstract]
  11. "Molecular characterization of human ninein protein: two distinct subdomains required for centrosomal targeting and regulating signals in cell cycle."Biochem. Biophys. Res. Commun. 308:975-983(2003) [PubMed] [Europe PMC] [Abstract]
  12. "A novel ninein-interaction protein, CGI-99, blocks ninein phosphorylation by GSK3beta and is highly expressed in brain tumors."FEBS Lett. 566:162-168(2004) [PubMed] [Europe PMC] [Abstract]
  13. "Autoantibodies to a group of centrosomal proteins in human autoimmune sera reactive with the centrosome."Arthritis Rheum. 41:551-558(1998) [PubMed] [Europe PMC] [Abstract]
  14. "Spectrum of centrosome autoantibodies in childhood varicella and post-varicella acute cerebellar ataxia."BMC Pediatr. 3:11-11(2003) [PubMed] [Europe PMC] [Abstract]
  15. "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
  16. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation."Int. J. Oncol. 37:429-436(2010) [PubMed] [Europe PMC] [Abstract]
  18. "DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome." Nature 456:66-72(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Novel microcephalic primordial dwarfism disorder associated with variants in the centrosomal protein ninein."J. Clin. Endocrinol. Metab. 97:E2140-E2151(2012) [PubMed] [Europe PMC] [Abstract]