Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS)

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HRS; ZFYVE8; Vps27; Protein pp110

Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS)
The deduced amino acid sequence of HGS, which the authors symbolized HRS, was found to be 93% homologous to that of mouse Hrs. HGS contains double zinc finger (FYVE) motifs, a putative coiled-coil sequence, and nucleotide-binding sites. Northern blot analysis detected expression of HGS in lymphoid and nonlymphoid tissues. Immunoblotting, however, determined that the phosphorylated species was found after but not before IL2 stimulation. Irrespective of IL2 stimulation, HGS coimmunoprecipitated with STAM, a signal-transducing adaptor molecule that is directly associated with and phosphorylated by JAK3 and JAK2 upon stimulation with IL2 and GMCSF, respectively. A mouse Hrs mutant from which the coiled-coil sequence was deleted lost STAM binding activity.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPH912Hu01 Recombinant Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) ELISA Kit Customized Service Offer
  1. "Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth."J. Biol. Chem. 272:32785-32791(1997) [PubMed] [Europe PMC] [Abstract]
  2. "Human Hrs, a tyrosine kinase substrate in growth factor-stimulated cells: cDNA cloning and mapping of the gene to chromosome 17."Gene 213:125-132(1998) [PubMed] [Europe PMC] [Abstract]
  3. "The neurofibromatosis 2 tumor suppressor protein interacts with hepatocyte growth factor-regulated tyrosine kinase substrate."Hum. Mol. Genet. 9:1567-1574(2000) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Hrs, a tyrosine kinase substrate with a conserved double zinc finger domain, is localized to the cytoplasmic surface of early endosomes."J. Biol. Chem. 272:20538-20544(1997) [PubMed] [Europe PMC] [Abstract]
  6. "The E3 ubiquitin ligase AIP4 mediates ubiquitination and sorting of the G protein-coupled receptor CXCR4."Dev. Cell 5:709-722(2003) [PubMed] [Europe PMC] [Abstract]
  7. "STAM and Hrs are subunits of a multivalent ubiquitin-binding complex on early endosomes."J. Biol. Chem. 278:12513-12521(2003) [PubMed] [Europe PMC] [Abstract]
  8. "HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein."J. Cell Biol. 162:425-434(2003) [PubMed] [Europe PMC] [Abstract]
  9. "Identification of human VPS37C, a component of endosomal sorting complex required for transport-I important for viral budding."J. Biol. Chem. 280:628-636(2005) [PubMed] [Europe PMC] [Abstract]
  10. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
  11. "A probability-based approach for high-throughput protein phosphorylation analysis and site localization."Nat. Biotechnol. 24:1285-1292(2006) [PubMed] [Europe PMC] [Abstract]
  12. "Hypertonia-associated protein Trak1 is a novel regulator of endosome-to-lysosome trafficking."J. Mol. Biol. 382:638-651(2008) [PubMed] [Europe PMC] [Abstract]
  13. "CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling."Mol. Biol. Cell 16:2470-2482(2005) [PubMed] [Europe PMC] [Abstract]
  14. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  15. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  16. "Membrane insertion of the FYVE domain is modulated by pH."Proteins 76:852-860(2009) [PubMed] [Europe PMC] [Abstract]
  17. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
  18. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  19. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  20. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  21. "Double-sided ubiquitin binding of Hrs-UIM in endosomal protein sorting."Nat. Struct. Mol. Biol. 13:272-277(2006) [PubMed] [Europe PMC] [Abstract]
  22. "DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome." Nature 456:66-72(2008) [PubMed] [Europe PMC] [Abstract]