Gamma-Glutamyl Hydrolase (gGH)

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GH; conjugase, folylpolygammaglutamyl hydrolase; Gamma-Glu-X carboxypeptidase

Gamma-Glutamyl Hydrolase (gGH)
GGH catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate.The cDNA encodes a 318-amino acid protein with a deduced amino acid sequence 67% identical to that of the rat enzyme. The N-terminal 24 residues are likely a leader sequence that mediates translocation of GGH into the endoplasmic reticulum for secretion. GGH also contains 4 potential N-glycosylation sites. Western blot analysis detected GGH at an apparent molecular mass of about 35 kD.human GGH showed higher activity toward the pentaglutamate derivative of methotrexate and had little activity against the diglutamate derivative. More than 60% of the total GGH activity was secreted into the medium of the 5 tumor cell lines examined.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPJ038Hu01 Recombinant Gamma-Glutamyl Hydrolase (gGH) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAJ038Hu01 Polyclonal Antibody to Gamma-Glutamyl Hydrolase (gGH) WB; IHC; ICC; IP.
MAJ038Hu21 Monoclonal Antibody to Gamma-Glutamyl Hydrolase (gGH) WB; IHC; ICC; IP.
Assay Kits SEJ038Hu ELISA Kit for Gamma-Glutamyl Hydrolase (gGH) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Gamma-Glutamyl Hydrolase (gGH) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Gamma-Glutamyl Hydrolase (gGH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Gamma-Glutamyl Hydrolase (gGH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Gamma-Glutamyl Hydrolase (gGH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Gamma-Glutamyl Hydrolase (gGH) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Gamma-Glutamyl Hydrolase (gGH) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Gamma-Glutamyl Hydrolase (gGH) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Gamma-Glutamyl Hydrolase (gGH) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Gamma-Glutamyl Hydrolase (gGH) CLIA Kit Customized Service Offer
n/a ELISA Kit for Gamma-Glutamyl Hydrolase (gGH) ELISA Kit Customized Service Offer
  1. "Human gamma-glutamyl hydrolase: cloning and characterization of the enzyme expressed in vitro."Proc. Natl. Acad. Sci. U.S.A. 93:10134-10138(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Structural organization of the human gamma-glutamyl hydrolase gene."Gene 238:463-470(1999) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Site-directed mutagenesis establishes cysteine-110 as essential for enzyme activity in human gamma-glutamyl hydrolase."Biochem. J. 343:551-555(1999) [PubMed] [Europe PMC] [Abstract]
  5. "Molecular modeling and site-directed mutagenesis define the catalytic motif in human gamma -glutamyl hydrolase."J. Biol. Chem. 275:40365-40370(2000) [PubMed] [Europe PMC] [Abstract]
  6. "Proteomic analysis of early melanosomes: identification of novel melanosomal proteins."J. Proteome Res. 2:69-79(2003) [PubMed] [Europe PMC] [Abstract]
  7. "Characterization of human gamma-glutamyl hydrolase in solution demonstrates that the enzyme is a non-dissociating homodimer."Biochim. Biophys. Acta 1764:1479-1486(2006) [PubMed] [Europe PMC] [Abstract]
  8. "Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes."J. Proteome Res. 5:3135-3144(2006) [PubMed] [Europe PMC] [Abstract]
  9. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  11. "Three-dimensional structure of human gamma -glutamyl hydrolase. A class I glutamine amidotransferase adapted for a complex substate."J. Biol. Chem. 277:24522-24529(2002) [PubMed] [Europe PMC] [Abstract]