SPARC Related Modular Calcium Binding Protein 1 (SMOC1)

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Secreted modular calcium-binding protein 1

SPARC Related Modular Calcium Binding Protein 1 (SMOC1)
The mature 408-amino acid protein contains a 26-residue signal peptide at the N terminus, followed by a follistatin-like domain, 2 thyroglobulin-like domains interrupted by a novel domain, and an extracellular calcium-binding domain. Recombinant expression in human cells showed that SMOC1 is a glycoprotein with a calcium-dependent conformation. Circular dichroism spectroscopy showed that SMOC1 has an approximately 40% beta-sheet structure. Northern blot analysis of adult mouse tissues revealed a 3.7-kb transcript with strong expression in ovary, and lower expression in brain, thymus, heart, skeletal muscle, liver, and lung. RT-PCR analysis detected a signal in testis also. Immunofluorescence studies showed that SMOC1 localized to basement membranes both in cultured cells and in mouse skin, kidney, and ovary.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPN564Hu01 Recombinant SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies n/a Monoclonal Antibody to SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Polyclonal Antibody Customized Service Offer
Assay Kits SEN564Hu ELISA Kit for SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SPARC Related Modular Calcium Binding Protein 1 (SMOC1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SPARC Related Modular Calcium Binding Protein 1 (SMOC1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to SPARC Related Modular Calcium Binding Protein 1 (SMOC1) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for SPARC Related Modular Calcium Binding Protein 1 (SMOC1) CLIA Kit Customized Service Offer
n/a ELISA Kit for SPARC Related Modular Calcium Binding Protein 1 (SMOC1) ELISA Kit Customized Service Offer
  1. "Characterization of SMOC-1, a novel modular calcium-binding protein in basement membranes."J. Biol. Chem. 277:37977-37986(2002) [PubMed] [Europe PMC] [Abstract]
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Secretome analysis of human BMSCs and identification of SMOC1 as an important ECM protein in osteoblast differentiation."J. Proteome Res. 9:2946-2956(2010) [PubMed] [Europe PMC] [Abstract]
  6. "SMOC1 is essential for ocular and limb development in humans and mice." Am. J. Hum. Genet. 88:30-41(2011) [PubMed] [Europe PMC] [Abstract]
  7. "Mutations in the SPARC-related modular calcium-binding protein 1 gene, SMOC1, cause waardenburg anophthalmia syndrome."Am. J. Hum. Genet. 88:92-98(2011) [PubMed] [Europe PMC] [Abstract]
  8. "Loss of the BMP antagonist, SMOC-1, causes Ophthalmo-acromelic (Waardenburg Anophthalmia) syndrome in humans and mice." PLoS Genet. 7:E1002114-E1002114(2011) [PubMed] [Europe PMC] [Abstract]
  9. "Mutations in ALDH1A3 cause Microphthalmia."Clin. Genet. 84:128-131(2013) [PubMed] [Europe PMC] [Abstract]