ELISA Kit for Fibrinogen Degradation Product (FDP)
FnDP; Fibrin Split Products; Fibrin Degradation Product
- Product No.CEA530Rb
- Organism SpeciesOryctolagus cuniculus (Rabbit) Same name, Different species.
- Sample Typeplasma
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range370.4-30000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 145.3ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Fibrinogen Degradation Product (FDP).
No significant cross-reactivity or interference between Fibrinogen Degradation Product (FDP) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Fibrinogen Degradation Product (FDP) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen Degradation Product (FDP) in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||80-91||87|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen Degradation Product (FDP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen Degradation Product (FDP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen Degradation Product (FDP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||78-101%||80-105%||82-96%||78-94%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
|Am J Physiol Lung Cell Mol Physiol||The role of the E2F1 transcription factor in the innate immune response to systemic LPS Physiology: Source|
|Bone Marrow Transplant.||Thrombomodulin alleviates murine GVHD in association with an increase in the proportion of regulatory T cells in the spleen Pubmed:25243628|
|Nephrology Dialysis Transplantation||The efficacy of recombinant human soluble thrombomodulin for the treatment of shiga toxin associated hemolytic uremic syndrome model mice Pubmed:25694534|
|Gastroenterology||Inhibition of Plasmin Protects Against Colitis in Mice by Suppressing Matrix Metalloproteinase 9–Mediated Cytokine Release From Myeloid Cells Sciencedirect:S0016508514014851|
|Gastroenterology||Inhibition of Plasmin Protects Against Colitis in Mice by PubMed: 25490065|
|Haematologica||Cytoprotective and pro-angiogenic functions of thrombomodulin are preserved in the C loop of the fifth epidermal growth factor-like domain Pubmed:29903766|
|Bone||Temporary inhibition of the plasminogen activator inhibits periosteal chondrogenesis and promotes periosteal osteogenesis during appendicular bone fracture … 10.1016:j.bone.2018.04.016|
|Thrombosis andHaemostasis||Nebulized anti-coagulants as a therapy for acute lung injury and acute respiratory distress syndrome Pubmed: 29202212|
|Critical care||Co-administration of iloprost and eptifibatide in septic shock (CO-ILEPSS)—a randomised, controlled, double-blind investigator-initiated trial investigating … Pubmed: 31488213|
|Journal of Thrombosis and Haemostasis||Effects of nebulized antithrombin and heparin on inflammatory and coagulation alterations in an acute lung injury model in rats Pubmed: 31755229|
|Catalog No.||Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species||Applications (RESEARCH USE ONLY!)|
|SEA530Rb||ELISA Kit for Fibrinogen Degradation Product (FDP)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|CEA530Rb||ELISA Kit for Fibrinogen Degradation Product (FDP)||Enzyme-linked immunosorbent assay for Antigen Detection.|