Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice

Cloud-Clone Corp.


On 14 January 2022, Christian Grimm, Ludwig-Maximilians-University, Munich, Germany, and his team published a paper tittled “Lung emphysema and impaired macrophage elastase clearance in mucolipin 3 deficient mice” in NATURE COMMUNICATIONS , which suggested that TRPML3 represents a key regulator of MMP-12 clearance by alveolar macrophages and may serve as therapeutic target for emphysema and chronic obstructive pulmonary disease.

 

The kits [ELISA Kit for Matrix Metalloproteinase 12 (MMP12), SEA402Mu] of Cloud-Clone brand was chosed in this article, we are so proud for supporting the reaserchers.


Lung emphysema and chronic bronchitis are the two most common causes of chronic obstructive pulmonary disease. Excess macrophage elastase MMP-12, which is predominantly secreted from alveolar macrophages, is known to mediate the development of lung injury and emphysema. Here, we discovered the endolysosomal cation channel mucolipin 3 (TRPML3) as a regulator of MMP-12 reuptake from broncho-alveolar fluid, driving in two independently generated Trpml3−/− mouse models enlarged lung injury, which is further exacerbated after elastase or tobacco smoke treatment. Mechanistically, using a Trpml3IRES-Cre/eR26-τGFP reporter mouse model, transcriptomics, and endolysosomal patch-clamp experiments, we show that in the lung TRPML3 is almost exclusively expressed in alveolar macrophages, where its loss leads to defects in early endosomal trafficking and endocytosis of MMP-12. Our findings suggest that TRPML3 represents a key regulator of MMP-12 clearance by alveolar macrophages and may serve as therapeutic target for emphysema and chronic obstructive pulmonary disease.