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Hypoxia Inducible Factor 1 Alpha (HIF1a)

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HIF1-A; MOP1; PASD8; Basic Helix-Loop-Helix Transcription Factor; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78

Hypoxia Inducible Factor 1 Alpha (HIF1a)
CCC A798
HGNC 4910
MGI 106918
PubMed 20864819
RGD 61928
UniProt Q16665
Wiki HIF1A

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. The hypoxia-inducible transcription factor 1α (HIF-1α) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.

HIF-1alpha abundance (and its subsequent activity) is regulated transcriptionally in an NF-κB dependent manner. In addition, the coordinated activity of the prolyl hydroxylases (PHDs) maintain the appropriate balance of HIF-1α protein in the post-translation phase.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Hu01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Hu02 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
APA798Hu02 Active Hypoxia Inducible Factor 1 Alpha (HIF1a) Cell culture; Activity Assays.
Antibodies PAA798Hu01 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC
MAA798Hu22 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC
LAA798Hu71 Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC.
LAA798Hu81 FITC-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IF.
PAA798Hu02 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC
MAA798Hu21 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC/IF
MAA798Hu23 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; ICC/IF
MAA798Hu24 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
MAA798Hu25 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
MAA798Hu27 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) IHC; ICC/IF
MAA798Hu29 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) IHC; ICC/IF
Assay Kits SEA798Hu ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA798Hu CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Chemiluminescent immunoassay for Antigen Detection.
HEA798Hu High Sensitive ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Mu01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Mu02 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA798Mu01 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
LAA798Mu71 Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC.
LAA798Mu81 FITC-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IF.
PAA798Mu02 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IP.
Assay Kits SEA798Mu ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA798Mu CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Ra01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Ra02 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA798Ra01 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC
PAA798Ra02 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) IHC
LAA798Ra71 Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC.
Assay Kits SEA798Ra ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA798Ra CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Chemiluminescent immunoassay for Antigen Detection.
HEA798Ra High Sensitive ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Oryctolagus cuniculus (Rabbit)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Rb01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA798Rb51 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IP.
MAA798Rb21 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IP.
Assay Kits SEA798Rb ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Sus scrofa; Porcine (Pig)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Po01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA798Po01 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) IHC
MAA798Po21 Monoclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IP.
Assay Kits SEA798Po ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA798Po CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Chemiluminescent immunoassay for Antigen Detection.

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Bo01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Bo02 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA798Bo01 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) IHC
LAA798Bo71 Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
PAA798Bo02 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; ICC/IF
Assay Kits SEA798Bo ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Chicken (Gallus)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA798Ga01 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Ga02 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
RPA798Ga03 Recombinant Hypoxia Inducible Factor 1 Alpha (HIF1a) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA798Ga01 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB; IHC; ICC; IP.
PAA798Ga02 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
PAA798Ga03 Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
LAA798Ga71 Biotin-Linked Polyclonal Antibody to Hypoxia Inducible Factor 1 Alpha (HIF1a) WB
Assay Kits n/a CLIA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) CLIA Kit Customized Service Offer
n/a ELISA Kit for Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit Customized Service Offer
  1. "Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension."Proc. Natl. Acad. Sci. U.S.A. 92:5510-5514(1995) [PubMed] [Europe PMC] [Abstract]
  2. "Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway."J. Biol. Chem. 272:8581-8593(1997) [PubMed] [Europe PMC] [Abstract]
  3. "The human hypoxia-inducible factor 1alpha gene: HIF1A structure and evolutionary conservation."Genomics 52:159-165(1998) [PubMed] [Europe PMC] [Abstract]
  4. "Preferential expression of the novel alternative isoform I.3 of hypoxia-inducible factor 1alpha in activated human T lymphocytes."Hum. Immunol. 69:421-425(2008) [PubMed] [Europe PMC] [Abstract]
  5. "The DNA sequence and analysis of human chromosome 14." Nature 421:601-607(2003) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "An essential role for p300/CBP in the cellular response to hypoxia."Proc. Natl. Acad. Sci. U.S.A. 93:12969-12973(1996) [PubMed] [Europe PMC] [Abstract]
  8. "Transactivation and inhibitory domains of hypoxia-inducible factor 1alpha. Modulation of transcriptional activity by oxygen tension."J. Biol. Chem. 272:19253-19260(1997) [PubMed] [Europe PMC] [Abstract]
  9. "Signal transduction in hypoxic cells: inducible nuclear translocation and recruitment of the CBP/p300 coactivator by the hypoxia-inducible factor-1alpha."EMBO J. 17:6573-6586(1998) [PubMed] [Europe PMC] [Abstract]
  10. "Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathway."Proc. Natl. Acad. Sci. U.S.A. 95:7987-7992(1998) [PubMed] [Europe PMC] [Abstract]
  11. "Molecular mechanisms of transcription activation by HLF and HIF1alpha in response to hypoxia: their stabilization and redox signal-induced interaction with CBP/p300."EMBO J. 18:1905-1914(1999) [PubMed] [Europe PMC] [Abstract]
  12. "Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1."Genes Dev. 13:64-75(1999) [PubMed] [Europe PMC] [Abstract]
  13. "Drosophila von Hippel-Lindau tumor suppressor complex possesses E3 ubiquitin ligase activity."Biochem. Biophys. Res. Commun. 276:355-361(2000) [PubMed] [Europe PMC] [Abstract]
  14. "Mechanism of regulation of the hypoxia-inducible factor-1 alpha by the von Hippel-Lindau tumor suppressor protein."EMBO J. 19:4298-4309(2000) [PubMed] [Europe PMC] [Abstract]
  15. "Redox-regulated recruitment of the transcriptional coactivators CREB-binding protein and SRC-1 to hypoxia-inducible factor 1alpha."Mol. Cell. Biol. 20:402-415(2000) [PubMed] [Europe PMC] [Abstract]
  16. "Hypoxia-inducible factor 1alpha protein expression is controlled by oxygen-regulated ubiquitination that is disrupted by deletions and missense mutations."Proc. Natl. Acad. Sci. U.S.A. 97:4748-4753(2000) [PubMed] [Europe PMC] [Abstract]
  17. "Independent function of two destruction domains in hypoxia-inducible factor-alpha chains activated by prolyl hydroxylation."EMBO J. 20:5197-5206(2001) [PubMed] [Europe PMC] [Abstract]
  18. "Binding and regulation of HIF-1alpha by a subunit of the proteasome complex, PSMA7."FEBS Lett. 498:62-66(2001) [PubMed] [Europe PMC] [Abstract]
  19. "Targeting of HIF-alpha to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation."Science 292:468-472(2001) [PubMed] [Europe PMC] [Abstract]
  20. "Regulation and destabilization of HIF-1alpha by ARD1-mediated acetylation."Cell 111:709-720(2002) [PubMed] [Europe PMC] [Abstract]
  21. "FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor."Genes Dev. 16:1466-1471(2002) [PubMed] [Europe PMC] [Abstract]
  22. "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor."Proc. Natl. Acad. Sci. U.S.A. 99:13459-13464(2002) [PubMed] [Europe PMC] [Abstract]
  23. "S-nitrosation of Cys-800 of HIF-1alpha protein activates its interaction with p300 and stimulates its transcriptional activity."FEBS Lett. 549:105-109(2003) [PubMed] [Europe PMC] [Abstract]
  24. "HIF-1 alpha protein as a target for S-nitrosation."FEBS Lett. 535:106-112(2003) [PubMed] [Europe PMC] [Abstract]
  25. "Structural basis for negative regulation of hypoxia-inducible factor-1alpha by CITED2."Nat. Struct. Biol. 10:504-512(2003) [PubMed] [Europe PMC] [Abstract]
  26. "Sumoylation increases HIF-1alpha stability and its transcriptional activity."Biochem. Biophys. Res. Commun. 324:394-400(2004) [PubMed] [Europe PMC] [Abstract]
  27. "Molecular cloning and characterization of the von Hippel-Lindau-like protein."Mol. Cancer Res. 2:43-52(2004) [PubMed] [Europe PMC] [Abstract]
  28. "VHL protein-interacting deubiquitinating enzyme 2 deubiquitinates and stabilizes HIF-1alpha."EMBO Rep. 6:373-378(2005) [PubMed] [Europe PMC] [Abstract]
  29. "Interaction between HIF-1 alpha (ODD) and hARD1 does not induce acetylation and destabilization of HIF-1 alpha."FEBS Lett. 579:6428-6432(2005) [PubMed] [Europe PMC] [Abstract]
  30. "Histone deacetylase inhibitors repress the transactivation potential of hypoxia-inducible factors independently of direct acetylation of HIF-alpha."J. Biol. Chem. 281:13612-13619(2006) [PubMed] [Europe PMC] [Abstract]
  31. "Clioquinol, a Cu(II)/Zn(II) chelator, inhibits both ubiquitination and asparagine hydroxylation of hypoxia-inducible factor-1alpha, leading to expression of vascular endothelial growth factor and erythropoietin in normoxic cells."J. Biol. Chem. 281:34056-34063(2006) [PubMed] [Europe PMC] [Abstract]
  32. "SUMOylation of hypoxia-inducible factor-1alpha reduces its transcriptional activity."Biochem. Biophys. Res. Commun. 360:646-652(2007) [PubMed] [Europe PMC] [Abstract]
  33. "RSUME, a small RWD-containing protein, enhances SUMO conjugation and stabilizes HIF-1alpha during hypoxia."Cell 131:309-323(2007) [PubMed] [Europe PMC] [Abstract]
  34. "RACK1 competes with HSP90 for binding to HIF-1alpha and is required for O(2)-independent and HSP90 inhibitor-induced degradation of HIF-1alpha."Mol. Cell 25:207-217(2007) [PubMed] [Europe PMC] [Abstract]
  35. "HIF-1alpha and EPAS ubiquitination mediated by the VHL tumour suppressor involves flexibility in the ubiquitination mechanism, similar to other RING E3 ligases."Oncogene 26:604-609(2007) [PubMed] [Europe PMC] [Abstract]
  36. "Transcriptional activation of HIF-1 by RORalpha and its role in hypoxia signaling."Arterioscler. Thromb. Vasc. Biol. 28:1796-1802(2008) [PubMed] [Europe PMC] [Abstract]
  37. "HIF-1 regulation: not so easy come, easy go."Trends Biochem. Sci. 33:526-534(2008) [PubMed] [Europe PMC] [Abstract]
  38. "Transcriptional regulation of hypoxia-inducible factor 1alpha by HIPK2 suggests a novel mechanism to restrain tumor growth."Biochim. Biophys. Acta 1793:368-377(2009) [PubMed] [Europe PMC] [Abstract]
  39. "HUMMR, a hypoxia- and HIF-1alpha-inducible protein, alters mitochondrial distribution and transport."J. Cell Biol. 185:1065-1081(2009) [PubMed] [Europe PMC] [Abstract]
  40. "Hypoxia-inducible protein 2 is a novel lipid droplet protein and a specific target gene of hypoxia-inducible factor-1."FASEB J. 24:4443-4458(2010) [PubMed] [Europe PMC] [Abstract]
  41. "Plk3 functions as an essential component of the hypoxia regulatory pathway by direct phosphorylation of HIF-1alpha."J. Biol. Chem. 285:38944-38950(2010) [PubMed] [Europe PMC] [Abstract]
  42. "Casein kinase 1 regulates human hypoxia-inducible factor HIF-1."J. Cell Sci. 123:2976-2986(2010) [PubMed] [Europe PMC] [Abstract]
  43. "UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1alpha accumulation."BMC Biol. 10:36-36(2012) [PubMed] [Europe PMC] [Abstract]
  44. "RSUME is implicated in HIF-1-induced VEGF-A production in pituitary tumour cells."Endocr. Relat. Cancer 19:13-27(2012) [PubMed] [Europe PMC] [Abstract]
  45. "Ubiquitin-specific protease 19 (USP19) regulates hypoxia-inducible factor 1alpha (HIF-1alpha) during hypoxia."J. Biol. Chem. 287:1962-1969(2012) [PubMed] [Europe PMC] [Abstract]
  46. "The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity."Nat. Cell Biol. 14:201-208(2012) [PubMed] [Europe PMC] [Abstract]
  47. "In silico structural and functional characterization of the RSUME splice variants."PLoS ONE 8:E57795-E57795(2013) [PubMed] [Europe PMC] [Abstract]
  48. "SIRT2 regulates tumour hypoxia response by promoting HIF-1alpha hydroxylation."Oncogene 34:1354-1362(2015) [PubMed] [Europe PMC] [Abstract]
  49. "A model for the complex between the hypoxia-inducible factor-1 (HIF-1) and its consensus DNA sequence."J. Biomol. Struct. Dyn. 18:169-179(2000) [PubMed] [Europe PMC] [Abstract]
  50. "Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha."J. Biol. Chem. 278:1802-1806(2003) [PubMed] [Europe PMC] [Abstract]
  51. "Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1 alpha."Proc. Natl. Acad. Sci. U.S.A. 99:5367-5372(2002) [PubMed] [Europe PMC] [Abstract]
  52. "Structural basis for Hif-1 alpha /CBP recognition in the cellular hypoxic response."Proc. Natl. Acad. Sci. U.S.A. 99:5271-5276(2002) [PubMed] [Europe PMC] [Abstract]
  53. "Structure of an HIF-1alpha-pVHL complex: hydroxyproline recognition in signaling."Science 296:1886-1889(2002) [PubMed] [Europe PMC] [Abstract]
  54. "Structural basis for the recognition of hydroxyproline in HIF-1 alpha by pVHL."Nature 417:975-978(2002) [PubMed] [Europe PMC] [Abstract]