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Otubain 1 (OTUB1)

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HSPC263; OTB1; OTU1; OTU Domain,Ubiquitin Aldehyde Binding 1; Deubiquitinating enzyme OTUB1; Ubiquitin-specific-processing protease OTUB1

Otubain 1 (OTUB1)
CCC L838
HGNC 23077
MGI 2147616
PubMed 22367539
RGD 1311329
UniProt Q96FW1
Wiki OTUB1
Otubain-1 is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains, being specific for lysine48 -linked polyubiquitin but not lysine63 -linked polyubiquitin. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system.
The 271-amino acid, 31-kD OTB1 protein contains UB-interaction motifs (UIMs) and UB-associated (UBA) domains, as well as putative nuclear localization signals and a consensus LxxLL motif. RT-PCR and Western blot analyses revealed wide expression of OTB1, with 2 isoforms in leukocytes.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL838Hu01 Recombinant Otubain 1 (OTUB1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL838Hu01 Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC; IP; FCM
LAL838Hu71 Biotin-Linked Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC.
LAL838Hu81 FITC-Linked Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC; IF.
Assay Kits n/a CLIA Kit for Otubain 1 (OTUB1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Otubain 1 (OTUB1) ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL838Mu01 Recombinant Otubain 1 (OTUB1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL838Mu01 Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC; IP.
LAL838Mu71 Biotin-Linked Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC.
LAL838Mu81 FITC-Linked Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC; IF.
Assay Kits n/a CLIA Kit for Otubain 1 (OTUB1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Otubain 1 (OTUB1) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPL838Ra01 Recombinant Otubain 1 (OTUB1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAL838Ra01 Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC; IP.
LAL838Ra71 Biotin-Linked Polyclonal Antibody to Otubain 1 (OTUB1) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Otubain 1 (OTUB1) CLIA Kit Customized Service Offer
n/a ELISA Kit for Otubain 1 (OTUB1) ELISA Kit Customized Service Offer
  1. "Otubains: a new family of cysteine proteases in the ubiquitin pathway."EMBO Rep. 4:517-522(2003) [PubMed] [Europe PMC] [Abstract]
  2. "Two isoforms of otubain 1 regulate T cell anergy via GRAIL."Nat. Immunol. 5:45-54(2004) [PubMed] [Europe PMC] [Abstract]
  3. "Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells." Genome Res. 10:1546-1560(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Human chromosome 11 DNA sequence and analysis including novel gene identification." Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  7. "Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family."Chem. Biol. 9:1149-1159(2002) [PubMed] [Europe PMC] [Abstract]
  8. "A quantitative atlas of mitotic phosphorylation."Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
  10. "OTU Domain-containing ubiquitin aldehyde-binding protein 1 (OTUB1) deubiquitinates estrogen receptor (ER) alpha and affects ERalpha transcriptional activity."J. Biol. Chem. 284:16135-16145(2009) [PubMed] [Europe PMC] [Abstract]
  11. "Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1."J. Mol. Biol. 386:1011-1023(2009) [PubMed] [Europe PMC] [Abstract]
  12. "Non-canonical inhibition of DNA damage-dependent ubiquitination by OTUB1."Nature 466:941-946(2010) [PubMed] [Europe PMC] [Abstract]
  13. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  14. "N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
  15. "OTU deubiquitinases reveal mechanisms of linkage specificity and enable ubiquitin chain restriction analysis."Cell 154:169-184(2013) [PubMed] [Europe PMC] [Abstract]
  16. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  17. "Structural basis and specificity of human otubain 1-mediated deubiquitination."Biochem. J. 418:379-390(2009) [PubMed] [Europe PMC] [Abstract]
  18. "OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function."Mol. Cell 45:384-397(2012) [PubMed] [Europe PMC] [Abstract]
  19. "The mechanism of OTUB1-mediated inhibition of ubiquitination."Nature 483:618-622(2012) [PubMed] [Europe PMC] [Abstract]