ELISA Kit for Glutathione (GSH)

L-Reduced Glutathione


This assay has high sensitivity and excellent specificity for detection of Glutathione (GSH).
No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.


Matrices listed below were spiked with certain level of Glutathione (GSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione (GSH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-105 87
EDTA plasma(n=5) 86-93 89
heparin plasma(n=5) 88-104 97


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione (GSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione (GSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione (GSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 90-102% 79-104% 97-105%
EDTA plasma(n=5) 94-103% 94-102% 97-104% 82-92%
heparin plasma(n=5) 94-101% 78-101% 90-97% 81-96%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
European Journal of Clinical Nutrition (2012) 66 Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants PubMed: 22781023
International Journal of Medical Sciences Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma PubMed: PMC3619117
Reproduction Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627
Iran J Basic Med Sci Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitation PubMed: 25810895
Bulletin of the Veterinary Institute in Pulawy Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird&#039;s Diet View: J
Journal of Allergy and Clinical Immunology Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress Pubmed:27016803
Oxidative Medicine and Cellular Longevity Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathway journals:2153745.pdf
Institutional repository of Vilnius University Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone induction object:elaba:15733325
Molecular Medicine Reports Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells pubmed:28260084
Molecular Medicine Reports Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells pubmed:29115483
Cancer Medicine Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic mice pubmed:28941178
Free radical research Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma … Pubmed:29463155
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336
Molecular Medicine Reports Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway Pubmed:29620209
Experimental and Therapeutic Medicine Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway Pubmed:30116359
Journal of Integrative Medicine Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats Doi: 10.1016/j.joim.2018.12.002
EXPERIMENTAL ANIMALS Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation Pubmed: 30606939
Acta Biomaterialia Porous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species … Pubmed: 30753941
Molecular Medicine Reports MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury Pubmed: 31115556
Earth and Environmental Science Germinated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milk
Frontiers in Neuroscience Hydrogen Sulfide Prevents Sleep Deprivation-Induced Hippocampal Damage by Upregulation of Sirt1 in the Hippocampus Pubmed: 32218719
Oxidative Medicine and Cellular Longevity Dexmedetomidine Attenuates Orthotopic Liver Transplantation-Induced Acute Gut Injury via α2-Adrenergic Receptor-Dependent Suppression of Oxidative Stress Pubmed: 31827710
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366
Experimental and Therapeutic Medicine Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy Pubmed: 33101467
BIOLOGICAL TRACE ELEMENT RESEARCH Association Between Serum Selenium Level and Subclinical Mastitis in Dairy Cattle Pubmed: 32583225
Toxicology Formaldehyde induces ferroptosis in hippocampal neuronal cells by upregulation of the Warburg effect 33259821
CELLULAR AND MOLECULAR LIFE SCIENCES Glutamine deficiency promotes stemness and chemoresistance in tumor cells through DRP1-induced mitochondrial fragmentation 33895866
Exp Ther Med Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats 34093766
Experimental and Therapeutic Medicine MicroRNA-145-5p aggravates cell apoptosis and oxidative stress in tongue squamous cell carcinoma 33732346
chemosphere Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective 33662726
Molecular Medicine Reports Lycium barbarum polysaccharides attenuate cardiovascular oxidative stress injury by enhancing the Keap1/Nrf2 signaling pathway in exhaustive exercise rats 34278476
J Healthc Eng κ-Opioid Receptor Agonist Ameliorates Postoperative Neurocognitive Disorder by Activating the Ca2+/CaMKII/CREB Pathway 34608407
Cell Death Discov Alamandine alleviates hypertension and renal damage via oxidative-stress attenuation in Dahl rats Pubmed:35022384
J Dairy Sci Intake of Bifidobacterium lactis Probio-M8 fermented milk protects against alcoholic liver disease Pubmed:35086715
Antioxidants Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice Pubmed:35740008
J Inflamm Res The κ-Opioid Receptor Agonist U50488H Ameliorates Neuropathic Pain Through the Ca2+/CaMKII/CREB Pathway in Rats Pubmed:35645576
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