ELISA Kit for Folic Acid (FA)

VB9; Vitamin B9; Folacin; Folate; V; Itamin M; Vitamin M; Vitamin Bc; Pteroyl-L-Glutamic Acid; Pteroyl-L-Glutamate


This assay has high sensitivity and excellent specificity for detection of Folic Acid (FA).
No significant cross-reactivity or interference between Folic Acid (FA) and analogues was observed.


Matrices listed below were spiked with certain level of Folic Acid (FA) and the recovery rates were calculated by comparing the measured value to the expected amount of Folic Acid (FA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 98
EDTA plasma(n=5) 79-90 86
heparin plasma(n=5) 80-89 84


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Folic Acid (FA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Folic Acid (FA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Folic Acid (FA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-93% 83-92% 98-105% 99-105%
EDTA plasma(n=5) 94-105% 97-105% 91-98% 78-90%
heparin plasma(n=5) 79-95% 79-94% 90-99% 93-103%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Annals of Surgical Oncology Vitamin Status and the Development of Postoperative Cognitive Decline in Elderly Surgical Oncologic Patients. pubmed:29058145
ACS Applied Materials & Interfaces Folic Acid-Functionalized Hybrid Photonic Barcodes for Capture and Release of Circulating Tumor Cells Pubmed:29882648
Journal of Pharmaceutical Sciences Development of Orally Applicable, Combinatorial Drug–Loaded Nanoparticles for the Treatment of Fibrosarcoma Pubmed:29339136
Aging Male Role of folic acid deficiency as a possible risk factor for erectile dysfunction Pubmed: 29157083
International Urology and Nephrology Low serum folic acid can be a potential independent risk factor for erectile dysfunction: a prospective case–control study Pubmed: 30547361
Int J Biol Macromol Enhanced cellular uptake, transport and oral bioavailability of optimized folic acid-loaded chitosan nanoparticles Pubmed:35292282
Frontiers in Nutrition Folate, Vitamin B12, and Homocysteine Levels in Women With Neural Tube Defect-Affected Pregnancy in Addis Ababa, Ethiopia Pubmed:35464038
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