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Multiplex Assay Kit for Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array)

LGALS8; PCTA1; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; Lectin,Galactoside-Binding Soluble 8; Po66 carbohydrate-binding protein; Prostate carcinoma tumor antigen 1

Specificity

This assay has high sensitivity and excellent specificity for detection of Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 82-97 89
heparin plasma(n=5) 95-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-99% 92-105% 98-105% 79-91%
EDTA plasma(n=5) 79-93% 80-101% 86-101% 82-96%
heparin plasma(n=5) 80-104% 96-103% 97-104% 81-89%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
Cell Metabolism Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer Pubmed: 31447322
CALCIFIED TISSUE INTERNATIONAL The Dysregulated Galectin Network Activates NF-κB to Induce Disease Markers and Matrix Degeneration in 3D Pellet Cultures of Osteoarthritic Chondrocytes Pubmed: 33185768
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPA308Bo01 Recombinant Galectin 8 (GAL8) Positive Control; Immunogen; SDS-PAGE; WB.
APA308Bo01 Active Galectin 8 (GAL8) Cell culture; Activity Assays.
PAA308Bo01 Polyclonal Antibody to Galectin 8 (GAL8) WB; IHC; ICC; IP.
MAA308Bo21 Monoclonal Antibody to Galectin 8 (GAL8) WB; IHC; ICC; IP.
SEA308Bo ELISA Kit for Galectin 8 (GAL8) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA308Bo Multiplex Assay Kit for Galectin 8 (GAL8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMA308Bo Multiplex Assay Kit for Galectin 8 (GAL8) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.