Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Multiplex Assay Kit for Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array)
ABP; TEBG; SBP; Sex steroid-binding protein; Testis-specific androgen-binding protein; Testosterone-estradiol-binding globulin; Testosterone-estrogen-binding globulin
- Product No.CMA396Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range1.9531-2000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.651pg/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 545
US$ 778
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 78-89 | 82 |
| EDTA plasma(n=5) | 86-93 | 89 |
| heparin plasma(n=5) | 78-93 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sex Hormone Binding Globulin (SHBG) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-102% | 80-97% | 94-101% | 80-102% |
| EDTA plasma(n=5) | 85-99% | 81-91% | 81-95% | 80-103% |
| heparin plasma(n=5) | 80-92% | 89-96% | 88-103% | 87-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:SHBG) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Nutrition and Cancer | A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers Tandfonline: Source |
| international journal of environmental research and public health | Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy PubMed: 25996886 |
| Journal of Life Science | Effects of Fermented Dandelion (Taraxacum coreanum) Extract on Male Climacteric Syndrome SMGHBM_2016_v26n9_1063 |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373 |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389 |
| Toxicology and Applied Pharmacology | Progressive effects of silver nanoparticles on hormonal regulation of reproduction in male rats pubmed:27746313 |
| 中国医药导报 | 益肾祛浊方对PCOS-IR大鼠Ghrelin、SHBG及VF的影响 yycyzx201733003 |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146 |
| Biomed Pharmacother | Comparative study on beneficial effects of vitamins B and D in attenuating doxorubicin induced cardiotoxicity in rats: Emphasis on calcium homeostasis 34029952 |
| Food Science and Biotechnology | Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats 33868755 |
| Nutrients | Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459 |