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Multiplex Assay Kit for Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array)

PPP3CA; PPP3-CA; CCN1; CNA1; CALN; CALNA1; PP2B; PP2-B; Protein Phosphatase 3(formerly 2B),Catalytic Subunit,Alpha Isoformprotein Phosphatase 2B; Calcineurin A Alpha

Specificity

This assay has high sensitivity and excellent specificity for detection of Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-99 93
EDTA plasma(n=5) 82-101 89
heparin plasma(n=5) 78-88 82

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-91% 78-89% 88-105% 97-104%
EDTA plasma(n=5) 92-99% 91-101% 90-102% 87-95%
heparin plasma(n=5) 84-102% 90-99% 91-103% 80-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Zaporozhye medical journal Кальциневрин як маркер г Handle: 123456789
american journal of transplantation Age-Dependent Metabolic and Immunosuppressive Effects of Tacrolimus. pubmed:27754593
Scientific Reports Differential protein expression of DARPP-32 versus Calcineurin in the prefrontal cortex and nucleus accumbens in schizophrenia and bipolar disorder Pubmed: 31619735
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RPB323Mu01 Recombinant Calcineurin (CaN) Positive Control; Immunogen; SDS-PAGE; WB.
SEB323Mu ELISA Kit for Calcineurin (CaN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB323Mu Multiplex Assay Kit for Calcineurin (CaN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMB323Mu Multiplex Assay Kit for Calcineurin (CaN) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.