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Multiplex Assay Kit for Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array)

FSCN1; SNL; p55; Singed-like protein; 55 kDa actin-bundling protein; Fascin homolog 1, Actin-Bundling Protein(Strongylocentrotus Purpuratus); Singed,Drosophila,Homolog-Like

Specificity

This assay has high sensitivity and excellent specificity for detection of Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-105 90
EDTA plasma(n=5) 83-90 86
heparin plasma(n=5) 96-104 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-99% 88-98% 78-98% 96-104%
EDTA plasma(n=5) 97-105% 85-94% 93-105% 84-98%
heparin plasma(n=5) 99-105% 86-103% 90-101% 86-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
Lung Cancer Serological investigation of the clinical significance of fascin in non-small-cell lung cancer ScienceDirect: S0169500213003826
American Journal of Translational Research Fascin-1 is released from proximal tubular cells in response to calcineurin inhibitors (CNIs) and correlates with isometric vacuolization in kidney transplanted patients PMC5622260
Genetics and Molecular Research Clinical significance of fascin-1 and laminin-5 in non-small cell lung cancer. pubmed:28653739
ЦИркУлИрУЮщИе актИН-СвЯЗЫваЮщИе БелкИ ПрИ ПроГреССИроваНИИ рака ГортаНИ И ГортаНоГлоткИ
Fascin-1 and its role as a serological marker in prostate cancer: a prospective case–control study 34737886
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB757Hu01 Recombinant Fascin (FSCN) Positive Control; Immunogen; SDS-PAGE; WB.
PAB757Hu01 Polyclonal Antibody to Fascin (FSCN) WB; IHC
FAB757Hu41 PE-Linked Anti-Fascin (FSCN) Polyclonal Antibody FCM
MAB757Hu22 Monoclonal Antibody to Fascin (FSCN) WB; IHC; ICC; IP.
SEB757Hu ELISA Kit for Fascin (FSCN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB757Hu Multiplex Assay Kit for Fascin (FSCN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMB757Hu Multiplex Assay Kit for Fascin (FSCN) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.