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Multiplex Assay Kit for Versican (VCAN) ,etc. by CBA (Cytometric Bead Array)

CSPG2; VS; ERVR; PG-M; WGN1; Chondroitin Sulfate Proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Glial hyaluronate-binding; Large fibroblast proteoglycan

Specificity

This assay has high sensitivity and excellent specificity for detection of Versican (VCAN) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 86-96 90
EDTA plasma(n=5) 83-91 88
heparin plasma(n=5) 86-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 79-88% 82-97% 90-97%
EDTA plasma(n=5) 82-96% 78-89% 86-94% 91-98%
heparin plasma(n=5) 78-98% 87-102% 82-94% 85-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
Plos one Tumor-Produced Versican V1 Enhances hCAP18/LL-37 Expression in Macrophages through Activation of TLR2 and Vitamin D3 Signaling to Promote Ovarian Cancer Progression In Vitro PubMed: PMC3570526
Clinica Chimica Acta Versican and its associated molecules: Potential diagnostic markers for multiple myeloma Pubmed:25623955
Journal of Cancer Research and Clinical Oncology Clinical significance of circulatory microRNA-203 in serum as novel potential diagnostic marker for multiple myeloma
RNA Biology Targeting of stromal versican by miR-144/199 inhibits multiple myeloma by downregulating FAK/STAT3 signaling Pubmed: 31532704
miR-124-3p and miR-181a-5p Mediate AT1-Receptor Autoantibody Induced Fetal Rat Cardiac Remodeling via Increased VCAN Expression
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB817Ra01 Recombinant Versican (VCAN) Positive Control; Immunogen; SDS-PAGE; WB.
PAB817Ra01 Polyclonal Antibody to Versican (VCAN) IHC
SEB817Ra ELISA Kit for Versican (VCAN) Enzyme-linked immunosorbent assay for Antigen Detection.
CMB817Ra Multiplex Assay Kit for Versican (VCAN) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
LMB817Ra Multiplex Assay Kit for Versican (VCAN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.