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Multiplex Assay Kit for Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array)
CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator
- Product No.CMB833Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.0391-40ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.013ng/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 385
US$ 550
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-93 | 86 |
| EDTA plasma(n=5) | 90-102 | 95 |
| heparin plasma(n=5) | 80-104 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Factor D (CFD) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-101% | 80-104% | 80-98% | 90-97% |
| EDTA plasma(n=5) | 94-102% | 78-94% | 97-104% | 92-101% |
| heparin plasma(n=5) | 97-105% | 78-96% | 89-98% | 88-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| Journal of Diabetes Investigation | Relationship between serum adipsin and the first phase of glucose‐stimulated insulin secretion in individuals with different glucose tolerance Pubmed:29432659 |
| Endocrine Practice | CORRELATION OF INCREASED SERUM ADIPSIN WITH INCREASED CARDIOVASCULAR RISKS IN ADULT PATIENTS WITH GROWTH HORMONE DEFICIENCY Pubmed: 30657359 |
| BMC Endocrine Disorders | Association between plasma adipsin level and mild cognitive impairment in Chinese patients with type 2 diabetes: a cross-sectional study Pubmed: 31651303 |
| Scientific Reports | HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse Pubmed: 33067533 |
| Biomedicine & Pharmacotherapy | Body fat distribution and circulating adipsin are related to metabolic risks in adult patients with newly diagnosed growth hormone deficiency and improve after … Pubmed: 33254428 |
| Nephrol Dial Transplant | Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis 33367879 |