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Multiplex Assay Kit for 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array)
11DH; 11-beta-HSD1; HDL; HSD11; HSD11B; HSD11L; SDR26C1; Corticosteroid 11-beta-dehydrogenase isozyme 1; Short Chain Dehydrogenase/Reductase Family 26C,Member 1
- Product No.CMC268Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.0977-100ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.0326ng/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 588
US$ 840
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-94 | 87 |
| EDTA plasma(n=5) | 87-95 | 91 |
| heparin plasma(n=5) | 93-101 | 97 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-101% | 96-104% | 78-99% | 78-101% |
| EDTA plasma(n=5) | 78-95% | 79-95% | 83-91% | 87-103% |
| heparin plasma(n=5) | 79-97% | 97-104% | 95-104% | 96-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| Journal of Endocrinology | Uninephrectomy reduces 11β-hydroxysteroid dehydrogenase type 1 and type 2 concomitantly with an increase in blood pressure in rats PubMed: 22739210 |
| Evid Based Complement Alternat Med | Anti-Inflammatory Property of PubMed: 26300946 |
| Molecules | Piper sarmentosum Effects on 11β-Hydroxysteroid Dehydrogenase Type 1 Enzyme in Serumand Bone in Rat Model of Glucocorticoid-Induced Osteoporosis. pubmed:27854305 |
| Bioorganic Chemistry | A novel derivatives of thiazol-4 (5H)-one and their activity in the inhibition of 11β-hydroxysteroid dehydrogenase type 1 Pubmed:29738970 |
| Malaysian Journal of Medical Sciences | An Evidence-Based Review: The Effects of Malaysian Traditional Herbs on Osteoporotic Rat Models |
| 2.583 | Application of ELISA Technique and Human Microsomes in the Search for 11β-Hydroxysteroid Dehydrogenase Inhibitors Doi: BioMed Research International |
| Chemistry & Biodiversity | Synthesis of the N‐methyl derivatives of 2‐aminothiazol‐4 (5H)‐one and their interactions with 11βHSD1‐molecular modeling and in vitro studies Pubmed: 31012543 |
| Oxidative Medicine and Cellular Longevity | Hepatoprotective Effects of Morinda citrifolia Leaf Extract on Ovariectomized Rats Fed with Thermoxidized Palm Oil Diet: Evidence at Histological and Ultrastructural … Pubmed: 31827717 |