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Multiplex Assay Kit for Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array)

FAS; OA519; SDR27X1; Short Chain Dehydrogenase/Reductase Family 27X,Member 1; S-acetyltransferase; 3-oxoacyl synthase; Oleoyl hydrolase; Enoyl-acyl-carrier-protein reductase

Specificity

This assay has high sensitivity and excellent specificity for detection of Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-104 92
EDTA plasma(n=5) 86-94 89
heparin plasma(n=5) 83-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 82-91% 79-99% 90-97% 96-103%
EDTA plasma(n=5) 91-98% 80-94% 84-92% 89-98%
heparin plasma(n=5) 90-98% 81-90% 98-105% 88-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
BMC Complementary and Alternative Medicine Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study Pubmed:25652121
Oncogenesis. Diet-induced alteration of fatty acid synthase in prostate cancer progression Pubmed:26878389
Animal Science Journal Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells pubmed:28804941
life sciences Clinical importance of FASN in relation to HIF-1α and SREBP-1c in gastric adenocarcinoma Pubmed: 30914315
Nutrients High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights …
bmc research notes The role of CA-125, GLS and FASN in predicting cytoreduction for epithelial ovarian cancers Pubmed: 32698888
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC470Mu02 Recombinant Fatty Acid Synthase (FASN) Positive Control; Immunogen; SDS-PAGE; WB.
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CMC470Mu Multiplex Assay Kit for Fatty Acid Synthase (FASN) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
LMC470Mu Multiplex Assay Kit for Fatty Acid Synthase (FASN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.