≡

Multiplex Assay Kit for Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array)

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

Specificity

This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-99 93
EDTA plasma(n=5) 90-104 98
heparin plasma(n=5) 93-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 79-97% 94-102% 91-104%
EDTA plasma(n=5) 86-99% 83-103% 92-101% 91-101%
heparin plasma(n=5) 81-103% 82-98% 83-94% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
AAPS PharmSciTech Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B-and siPDGFR-β-Containing Chitosan Nanoplexes pubmed:27975193
Marmara Pharmaceutical Journal In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes 5000184338
International Journal of Clinical and Experimental Pathology Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens ISSN:1936-2625/IJCEP0058424
Cell Biology International Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathway Pubmed: 31535749
Ann Agric Environ Med PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis Pubmed: 32208584
Journal of Tissue Engineering Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders 33868627
Catalog No. Related products for research use of Bos taurus; Bovine (Cattle) Organism species Applications (RESEARCH USE ONLY!)
RPC921Bo01 Recombinant Platelet Derived Growth Factor Subunit B (PDGFB) Positive Control; Immunogen; SDS-PAGE; WB.
SEC921Bo ELISA Kit for Platelet Derived Growth Factor Subunit B (PDGFB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC921Bo Multiplex Assay Kit for Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMC921Bo Multiplex Assay Kit for Platelet Derived Growth Factor Subunit B (PDGFB) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.