≡

Multiplex Assay Kit for Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array)

Homovanilic Acid; HOC6H3(OCH3)CH2COOH; 3-Methoxy-4-hydroxyphenyl Acetic Acid; 4-Hydroxy-3-Methoxy-Benzeneacetic Acid; 4-Hydroxy-3-Methoxyphenylacetic Acid

Specificity

This assay has high sensitivity and excellent specificity for detection of Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 91-104 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-94% 78-89% 85-95% 86-97%
EDTA plasma(n=5) 90-98% 82-89% 90-98% 92-101%
heparin plasma(n=5) 94-105% 98-105% 95-103% 93-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:HVA) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Psychopharmacology Ningdong granule: a complementary and alternative therapy in the treatment of attention deficit/hyperactivity disorder PubMed: 21416235
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients PubMed: 25926735
Evidence-Based Complementary and Alternative Medicine Ningdong Granule Upregulates the Striatal DA Transporter and Attenuates Stereotyped Behavior of Tourette Syndrome in Rats Pubmed: 33005197
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPD717Ge11 BSA Conjugated Homovanillic Acid (HVA) Immunogen; SDS-PAGE; WB.
CPD717Ge21 OVA Conjugated Homovanillic Acid (HVA) Immunogen; SDS-PAGE; WB.
PAD717Ge01 Polyclonal Antibody to Homovanillic Acid (HVA) WB; IHC; ICC; IP.
CED717Ge ELISA Kit for Homovanillic Acid (HVA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD717Ge Multiplex Assay Kit for Homovanillic Acid (HVA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMD717Ge Multiplex Assay Kit for Homovanillic Acid (HVA) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
KSD717Ge11 ELISA Kit DIY Materials for Homovanillic Acid (HVA) Main materials for "Do It (ELISA Kit) Yourself".