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Multiplex Assay Kit for Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array)

EPLG5; HTKL; Htk-L; LERK5; HTK Ligand; Ligand Of Eph-Related Kinase 5; Eph-Related Receptor Tyrosine Kinase Ligand 5

Specificity

This assay has high sensitivity and excellent specificity for detection of Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 99
EDTA plasma(n=5) 94-104 99
heparin plasma(n=5) 91-99 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 96-103% 91-105% 90-104% 93-102%
EDTA plasma(n=5) 79-102% 97-105% 80-101% 93-104%
heparin plasma(n=5) 87-101% 82-94% 83-96% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity

Assay procedure summary

1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.

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Magazine Citations
ATS Journals Soluble Ephrin-B2 is a Novel Biomarker for Idiopathic Pulmonary Fibrosis Doi: Pdf
D50. PEDIATRIC RARE LUNG DISEASES Soluble Ephrin-B2 Is A Novel Biomarker Of Pulmonary Fibrosis doi:A7217
An International Journal of Medicine  P055 Over-expression of IL-6 and OSM impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis content:109
An International Journal of Medicine P057 Soluble Ephrin-B2 is a Prognostic Biomarker of Pulmonary Fibrosis S40.2.extract
4 ADAM10-mediated ephrin-B2 shedding promotes myofibroblast activation and organ fibrosis pubmed:29058717
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE112Hu02 Recombinant Ephrin B2 (EFNB2) Positive Control; Immunogen; SDS-PAGE; WB.
APE112Hu02 Active Ephrin B2 (EFNB2) Cell culture; Activity Assays.
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PAE112Hu01 Polyclonal Antibody to Ephrin B2 (EFNB2) IHC
MAE112Hu21 Monoclonal Antibody to Ephrin B2 (EFNB2) WB; IHC; ICC; IP.
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CME112Hu Multiplex Assay Kit for Ephrin B2 (EFNB2) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.