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Multiplex Assay Kit for Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array)
REG-III; PAP1B; PAPIB; UNQ429; Pancreatitis-associated protein 1B; Regenerating islet-derived protein III-gamma
- Product No.CME676Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range1.9531-2000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.651pg/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 605
US$ 864
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 94-101 | 97 |
| EDTA plasma(n=5) | 88-102 | 94 |
| heparin plasma(n=5) | 95-102 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Regenerating Islet Derived Protein 3 Gamma (REG3g) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-94% | 88-95% | 79-99% | 93-101% |
| EDTA plasma(n=5) | 80-101% | 81-97% | 97-105% | 91-99% |
| heparin plasma(n=5) | 78-95% | 92-101% | 86-101% | 79-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| 16 | Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumonia PubMed: PMC3600913 |
| Biology of Blood and Marrow Transplatation | Reciprocal Expression of Enteric Antimicrobial Proteins in Intestinal Graft-Versus-Host Disease Pubmed: 23927965 |
| Nature Comunication | Hyperglycaemia inhibits REG3A expression to exacerbate TLR3-mediated skin inflammation in diabetes. pubmed:27830702 |
| Gastroenterology | Enteric Delivery of Regenerating Family Member 3 alpha Alters the Intestinal Microbiota and Controls Inflammation in Mice With Colitis Pubmed:29133078 |
| Kidney International | Interleukin-6/Stat3 signaling has an essential role in the host antimicrobial response to urinary tract infection Pubmed:29475562 |
| Journal of Immunology Research | Role of TLR5 in the Translocation and Dissemination of Commensal Bacteria in the Intestine after Traumatic Hemorrhagic Shock 34840990 |