ELISA Kit DIY Materials for Immunoglobulin G4 (IgG4)

IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

  • Product No.KSA234Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.075ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1134  US$ 1890  US$ 3402  US$ 6615  US$ 11340 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Immunoglobulin G4 (IgG4).No significant cross-reactivity or interference between Immunoglobulin G4 (IgG4) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Oncology Reports Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancer Pubmed:25529861
Iranian Journal of Pharmaceutical Research Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 Cells Pubmed:25561924
Ann Hematol Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s disease PubMed: 26377996
Biomed Res Int The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling PubMed: 26161394
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KSA234Hu01 ELISA Kit DIY Materials for Immunoglobulin G4 (IgG4) Main materials for "Do It (ELISA Kit) Yourself".