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ELISA Kit DIY Materials for Aggrecan (AGC)

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

  • Product No.KSB908Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range1.56-100ng/mL
  • Applicable Sensitivity0.59ng/mL
  • ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
  • DownloadInstruction Manual
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1197  US$ 1995  US$ 3591  US$ 6983  US$ 11970 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of  Aggrecan (AGC). No significant cross-reactivity or interference between  Aggrecan (AGC) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells Rsmjournals: Source
Age( dordr). In vitro tenocyte metabolism in aging and oestrogen deficiency Springer: Source
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Stem Cell Research & Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. Pubmed: 24739658
Materials Science and Engineering: C Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition ScienceDirect: S0928493113006656
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes Pubmed:25333747
Macromolecular Bioscience Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration PubMed: 25787871
Osteoarthritis and Cartilage Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs Pubmed:26802547
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes Pubmed:27090063
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. pubmed:28187731
Journal of Clinical Medicine Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors Pubmed: 32392807
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs
researchsquare Aggrecan-a new biomarker for acute type A aortic dissection 33990642
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB908Hu02 Recombinant Aggrecan (AGC) Positive Control; Immunogen; SDS-PAGE; WB.
RPB908Hu01 Recombinant Aggrecan (AGC) Positive Control; Immunogen; SDS-PAGE; WB.
RPB908Hu03 Recombinant Aggrecan (AGC) Positive Control; Immunogen; SDS-PAGE; WB.
PAB908Hu02 Polyclonal Antibody to Aggrecan (AGC) WB; IHC
PAB908Hu03 Polyclonal Antibody to Aggrecan (AGC) WB; IHC; ICC; IP.
PAB908Hu01 Polyclonal Antibody to Aggrecan (AGC) ICC/IF
LAB908Hu81 FITC-Linked Polyclonal Antibody to Aggrecan (AGC) WB; IHC; ICC; IF.
LAB908Hu71 Biotin-Linked Polyclonal Antibody to Aggrecan (AGC) WB; IHC; ICC.
MAB908Hu21 Monoclonal Antibody to Aggrecan (AGC) WB; IHC; ICC; IP.
MAB908Hu22 Monoclonal Antibody to Aggrecan (AGC) WB
SEB908Hu ELISA Kit for Aggrecan (AGC) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB908Hu CLIA Kit for Aggrecan (AGC) Chemiluminescent immunoassay for Antigen Detection.
LMB908Hu Multiplex Assay Kit for Aggrecan (AGC) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB908Hu01 ELISA Kit DIY Materials for Aggrecan (AGC) Main materials for "Do It(ELISA Kit) Yourself"