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ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF)

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

  • Product No.KSB972Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.156-10ng/mL
  • Applicable Sensitivity0.059ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1300  US$ 2166  US$ 3899  US$ 7581  US$ 12996 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF). No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Molecular Therapy A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions Nature: 2010144
American Journal of Nephrology Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy Karger: 314326
34 Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury OxfordJournals: source
Heart and Education in Heart The change of serum level of pigment epithelium-derived factor in coronary heart disease Bmj: Source
Mediators of Inflammation Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation Pubmed: 24288442
Cardiology. The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. Pubmed: 24192856
Endocrinology Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism Endocrine: Source
Clinical Science Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis PubMed: 25700221
Molecular Sciences Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R Pubmed:27187377
Investigative ophthalmoloey & visual science The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells articleid:2582822
Scientific reports VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice pubmed:28091556
国际眼科杂志 色素上皮衍生因子修饰的人脐带间充质干细胞构建方法 201707007
Investigative Ophthalmology & Visual Science PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice Pubmed:30025136
International Eye Science 高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响 2018/2/201802020.pdf
Domestic Animal Endocrinology Lactation-related changes in tissue expression of PEDF in dairy cows Pubmed:29758402
Medical Science Monitor Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a … Pubmed:29574467
Chemosphere Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis Doi: 10.1016/j.chemosphere.2018.10.100
Biological Trace Element Research Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model Pubmed: 30392020
International Urology and Nephrology Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement Pubmed: 30536192
International Journal of Ophthalmology A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807
Journal of the American Heart Association Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia Pubmed: 31711388
environmental research Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells Pubmed: 32315827
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells 33498962
Hum Cell Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡­ 33768511
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB972Ra01 Recombinant Pigment Epithelium Derived Factor (PEDF) Positive Control; Immunogen; SDS-PAGE; WB.
PAB972Ra01 Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF) IHC
MAB972Ra21 Monoclonal Antibody to Pigment Epithelium Derived Factor (PEDF) WB; IHC; ICC; IP.
SEB972Ra ELISA Kit for Pigment Epithelium Derived Factor (PEDF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB972Ra CLIA Kit for Pigment Epithelium Derived Factor (PEDF) Chemiluminescent immunoassay for Antigen Detection.
LMB972Ra Multiplex Assay Kit for Pigment Epithelium Derived Factor (PEDF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB972Ra01 ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF) Main materials for "Do It (ELISA Kit) Yourself".