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ELISA Kit DIY Materials for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5)

ADMP-2; Aggrecanase-2

  • Product No.KSK205Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.112ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1260  US$ 2100  US$ 3780  US$ 7350  US$ 12600 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5). No significant cross-reactivity or interference between A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source
Osteoarthritis and Cartilage Lack of anti-inflammatory and anti-catabolic effects on basal inflamed osteoarthritic chondrocytes or synoviocytes by adipose stem cell-conditioned medium PubMed: 26521751
Journal of Cellular and Molecular Medicine VIP and CRF reduce ADAMTS expression and function in osteoarthritis synovial fibroblasts Pubmed:26818776
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. Pubmed:26991748
The American journal of pathology Healthy and Osteoarthritic Synovial Fibroblasts Produce a Disintegrin and Metalloproteinase with Thrombospondin Motifs 4, 5, 7, and 12: Induction by IL-1β and Fibronectin and Contribution to Cartilage Damage. pubmed:27449198
PLoS One. Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders. pubmed:28207788
PLoS One Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders 10.1371:journal.pone.0172181
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases pubmed:26991748
BMC musculoskeletal disorders Hemoglobin stimulates the expression of ADAMTS-5 and ADAMTS-9 by synovial cells: a possible cause of articular cartilage damage after intra-articular hemorrhage. pubmed:29137610
Journal of Clinical Medicine The Effects of TNF-α Inhibition on the Metabolism of Cartilage: Relationship between KS, HA, HAPLN1 and ADAMTS4, ADAMTS5, TOS and TGF-β1 Plasma … Pubmed:35407621
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPK205Hu01 Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) Positive Control; Immunogen; SDS-PAGE; WB.
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MAK205Hu21 Monoclonal Antibody to A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) WB; IHC; ICC; IP.
SEK205Hu ELISA Kit for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMK205Hu Multiplex Assay Kit for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSK205Hu01 ELISA Kit DIY Materials for A Disintegrin And Metalloproteinase With Thrombospondin 5 (ADAMTS5) Main materials for "Do It (ELISA Kit) Yourself".