Magnetic Luminex Assay Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc.
CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA116Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 1Plex2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
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This assay has high sensitivity and excellent specificity for detection of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc..
No significant cross-reactivity or interference between Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc. and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc. in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|96-well plate||1||Plate sealer for 96 wells||4|
|Pre-Mixed Standard||2||Standard Diluent||1×20mL|
|Pre-Mixed Magnetic beads (22#:RANTES)||1||Analysis buffer||1×20mL|
|Pre-Mixed Detection Reagent A||1×120μL||Assay Diluent A||1×12mL|
|Detection Reagent B (PE-SA)||1×120μL||Assay Diluent B||1×12mL|
|Sheath Fluid||1×10mL||Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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