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Multiplex Assay Kit for Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay)
HMIWC2; MIWC; WCH4; Mercurial-insensitive water channel
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA582Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.0195-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.0065ng/mL.
- Downloadn/a
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 393
US$ 408
US$ 431
US$ 461
US$ 491
US$ 537
US$ 605
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Result
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 101 |
| EDTA plasma(n=5) | 80-89 | 83 |
| heparin plasma(n=5) | 81-101 | 89 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aquaporin 4 (AQP4) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-105% | 83-90% | 83-90% | 79-89% |
| EDTA plasma(n=5) | 78-99% | 90-97% | 94-105% | 88-102% |
| heparin plasma(n=5) | 83-91% | 80-96% | 94-101% | 85-93% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:AQP4) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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| Magazine | Citations |
| Neuroscience Letters | Aquaporins AQP1 and AQP4 in the cerebrospinal fluid of bacterial meningitis patients ScienceDirect: S0304394011012341 |
| Neurology | Astrocytic damage is far more severe than demyelination in NMO Neurology: 208 |
| Fluids and Barriers of the CNS | Aquaporin-4 expression in the cerebrospinal fluid in congenital human hydrocephalus PubMed: PMC3651869 |
| Neuropeptides | Melanocortin MC4 receptor agonists alleviate brain damage in abdominal compartment syndrome in the rat PubMed: 25616531 |
| Human & Experimental Toxicology | Novel effect of Daflon and low-dose γ-radiation in modulation of thioacetamide-induced hepatic encephalopathy in male albino rats Pubmed:26987350 |
| Cephalalgia | Decreased levels of aquaporin-4 in the cerebrospinal fluid of patients with idiopathic intracranial hypertension Pubmed:26853804 |
| PLoS One | Cerebrospinal fluid biomarkers of infantile congenital hydrocephalus. pubmed:28212403 |
| Journal of Neurotrauma | Neuron-derived Plasma Exosome Proteins after Remote Traumatic Brain Injury Pubmed: 31441374 |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease 33995926 |
| PLoS One | Evaluation of aquaporins in the cerebrospinal fluid in patients with idiopathic normal pressure hydrocephalus 34597351 |