Magnetic Luminex Assay Kit for Thioredoxin (Trx) ,etc.
TXN; ADF; SASP; TRDX; TRX1; ATL-derived factor; Surface-associated sulphydryl protein
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA702Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range1.95-2000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.65 pg/mL.
- DownloadInstruction Manual
- UOM 1Plex2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
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This assay has high sensitivity and excellent specificity for detection of Thioredoxin (Trx) ,etc..
No significant cross-reactivity or interference between Thioredoxin (Trx) ,etc. and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Thioredoxin (Trx) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Thioredoxin (Trx) ,etc. in samples.
|Matrix||Recovery range (%)||Average(%)|
|sodium citrate plasma(n=5)||78-102||91|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin (Trx) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin (Trx) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Thioredoxin (Trx) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|sodium citrate plasma(n=5)||78-103%||83-101%||81-90%||79-102%|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|96-well plate||1||Plate sealer for 96 wells||4|
|Pre-Mixed Standard||2||Standard Diluent||1×20mL|
|Pre-Mixed Magnetic beads (22#:Trx)||1||Analysis buffer||1×20mL|
|Pre-Mixed Detection Reagent A||1×120μL||Assay Diluent A||1×12mL|
|Detection Reagent B (PE-SA)||1×120μL||Assay Diluent B||1×12mL|
|Sheath Fluid||1×10mL||Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
|Human Reproduction||The roles of thioredoxin and thioredoxin-binding protein-2 in endometriosis OxfordJournals: source|
|Exp Ther Med.||Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus Pubmed:Pmc4061188|
|Reproduction||Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627|
|Free Radical Research||Thalassemia major patients using iron chelators showed a reduced plasma thioredoxin level and reduced thioredoxin reductase activity, despite elevated oxidative stress Pubmed:25564095|
|Antioxidants||Comparison of the Pulmonary Oxidative Stress Caused by Intratracheal Instillation and Inhalation of NiO Nanoparticles when Equivalent Amounts of NiO Are Retained in the Lung. Pubmed:26797643|
|FASEB Journal||Relationship between aortic wall oxidative stress/proteolytic enzyme expression and intraluminal thrombus thickness indicates a novel pathomechanism in the … Pubmed: 30351992|
|World Journal of Gastrointestinal Oncology||Redox, immune and genetic biomarker system for personalized treatments in colorectal cancer Doi: 10.4251/wjgo.v11.i2.117|
|Nutrients||The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479|
|European Journal of Pharmacology||Nilotinib enhances β-islets integrity and secretory functions in a rat model of STZ-induced diabetes mellitus Pubmed: 31351037|
|Journal of Clinical Medicine||Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort Pubmed: 32210109|
|HISTOCHEMISTRY AND CELL BIOLOGY||Thioredoxin 1 is upregulated in the bone and bone marrow following experimental myocardial infarction: evidence for a remote organ response Pubmed: 33161477|
|Journal of Cosmetic Dermatology||Examination of androgenetic alopecia with serum biomarkers Pubmed: 32969583|
|Heliyon||Hepatoprotective Effect of Saccharomyces Cervisciae Cell Wall Extract against Thioacetamide-induced Liver Fibrosis in Rats 34159266|
|Hum Exp Toxicol||Carvacrol hinders the progression of hepatic fibrosis via targeting autotaxin and thioredoxin in thioacetamide-induced liver fibrosis in rat 34155936|
|Catalog No.||Related products for research use of Mus musculus (Mouse) Organism species||Applications (RESEARCH USE ONLY!)|
|RPA702Mu02||Recombinant Thioredoxin (Trx)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA702Mu01||Recombinant Thioredoxin (Trx)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA702Mu01||Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC; IP.|
|PAA702Mu02||Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC; IP.|
|LAA702Mu81||FITC-Linked Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC; IF.|
|LAA702Mu71||Biotin-Linked Polyclonal Antibody to Thioredoxin (Trx)||WB; IHC; ICC.|
|SEA702Mu||ELISA Kit for Thioredoxin (Trx)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|LMA702Mu||Magnetic Luminex Assay Kit for Thioredoxin (Trx) ,etc.||Magnetic Luminex Assay for Antigen Detection.|