Multiplex Assay Kit for Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay)

TIG2; RARRES2; Tazarotene-Induced Gene 2; Retinoic Acid Receptor Responder 2,Tazarotene Induced; RAR-responsive protein TIG2

(Note: Up to 8-plex in one testing reaction)


This assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-90 80
EDTA plasma(n=5) 78-89 83
heparin plasma(n=5) 80-95 85


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemerin (CHEM) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-90% 82-104% 78-88% 97-105%
EDTA plasma(n=5) 87-95% 91-99% 92-101% 89-96%
heparin plasma(n=5) 98-105% 88-97% 88-95% 83-97%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:CHEM) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.



Magazine Citations
Scandinavian Journal of Gastroenterology Serum chemerin and vaspin in non-alcoholic fatty liver disease PubMed: 20095887
Gastroenterology & Hepatology Chemerin, vaspin and insulin resistance in chronic hepatitis C Wiley: source
Placenta Expression and regulation of chemerin during rat pregnancy ScienceDirect: S0143400412000902
Acta Pharmacologica Sinica Chronic ethanol consumption increases the levels of chemerin in the serum and adipose tissue of humans and rats Nature: 201211
RAED dergisi Ankilozan spondilitli hastalarda adipositokin düzeyleri ve insülin direnci Raeddergisi: Source
Journal of Cellular and Molecular Medicine Association of chemerin with oxidative stress, inflammation and classical adipokines in non-diabetic obese patients Pubmed: 24702860
Clinical Endocrinology Strong correlations between circulating chemerin levels and lipoprotein subfractions in nondiabetic obese and nonobese subjects Pubmed: 24303851
Oral Dis. Impact of OSA on Biological Markers in Morbid Obesity and Metabolic Syndrome Pubmed:24106991
Endocrine Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia Pubmed:24840719
The Clinical Respiratory Journal Relationship between plasma chemerin levels and disease severity in COPD patients Pubmed:24865134
Gynecol Endocrinol. Elevated serum chemerin in Chinese women with hyperandrogenic PCOS Pubmed:24927079
Endocrine. Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats. Pubmed:25471281
Obesity Surgery Long-term Effect of Ileal Transposition on Adipokine Serum Level in Zucker (Orl)-Lepr fa Fatty Rats Pubmed:25697126
Dermatologica Sinica Chemerin and calprotectin levels correlate with disease activity and inflammation markers in psoriasis vulgaris Sciencedirect:S1027811714000470
Dis Markers Serum Chemerin Levels in relation to Osteoporosis and Bone Mineral Density: A Case-Control Study PubMed: 26170530
Med Sci Monit Association of Serum Chemerin Levels with Acute Ischemic Stroke and Carotid Artery Atherosclerosis in a Chinese Population PubMed: 26471865
Endocrine Protective effect of liraglutide against ER stress in the liver of high-fat diet-induced insulin-resistant rats PubMed: 25471281
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Effects of alpha-lipoic acid on chemerin secretion in 3T3-L1 and human adipocytes Pubmed:26721419
Medicine (Baltimore). Association of chemerin levels and bone mineral density in Chinese obese postmenopausalwomen. pubmed:27583869
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … Pubmed:29849871
Current Eye Research Chemerin is associated with diabetic retinopathy in type 2 diabetes Pubmed:25848840
Journal of Oral Rehabilitation Expression of chemerin in the synovial fluid of patients with temporomandibular joint disorders Pubmed:29331039
International Journal of Cancer Epigenetic regulation of Amphiregulin and Epiregulin in colorectal cancer Pubmed: 30252132
Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats Pubmed: 30292771
Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-κB signal Doi: 10.1016/j.pan.2019.02.005
Hypertens Pregnancy Protective effect of vitamin D supplementation in a rat modal of preeclampsia: a possible implication of chemerin Pubmed: 30922121
Pancreatology Chemerin alleviates acute pancreatitis in the rat thorough modulation of NF-kB signal Pubmed: 30833212
Digestion Association between High Levels of Circulating Chemerin and Colorectal Adenoma in Men Pubmed: 31269491
Vitamin D Supplementation Reduces Serum Chemerin Level in Gestational Diabetes Mellitus Rat Model
Aging Medicine Plasma levels of adiponectin and chemerin are associated with early stage of atherosclerosis in older adults with type 2 diabetes mellitus
Eur J Nutr The dietary inflammatory index is associated with anti-and pro-inflammatory adipokines in Brazilian schoolchildren 33575861
Eur J Pediatr Pro-and anti-inflammatory adipokines are associated with cardiometabolic risk markers in Brazilian schoolchildren 33834274
Indian Journal of Forensic Medicine & Toxicology Altered Serum Marker of Adipokines Profile in Breast Cancer Women
Int J Womens Health Association of High Serum Chemerin with Bone Mineral Density Loss and Osteoporotic Fracture in Elderly Chinese Women Pubmed:35140527
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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