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Multiplex Assay Kit for Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) ,etc. by FLIA (Flow Luminescence Immunoassay)
C14orf141; LTBP3
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB630Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.02-20ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.007 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
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Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2).
No significant cross-reactivity or interference between Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) and the recovery rates were calculated by comparing the measured value to the expected amount of Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-101 | 87 |
| EDTA plasma(n=5) | 78-91 | 83 |
| heparin plasma(n=5) | 82-92 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 82-91% | 98-105% | 92-99% | 87-101% |
| EDTA plasma(n=5) | 87-105% | 87-103% | 78-89% | 96-103% |
| heparin plasma(n=5) | 97-105% | 85-96% | 87-94% | 96-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:LTBP2) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| International Journal of Cancer | Osteopontin and latent-TGF β binding-protein 2 as potential diagnostic markers for HBV-related hepatocellular carcinoma Pubmed:24803312 |
| Int J Cancer | Osteopontin and latent‐TGF β binding‐protein 2 as potential diagnostic markers for HBV‐related hepatocellular carcinoma PubMed: 24803312 |
| Biology of Blood and Marrow Transplantation | Association of Plasma CD163 Concentration with De Novo–Onset Chronic Graft-versus-Host Disease pubmed:28455006 |
| Biology of Blood and Marrow Transplantation | Association of plasma CD163 concentration with de novo-onset chronic graft-versus-host disease 10.1016:j.bbmt.2017.04.019 |
| Clinical Science | LTBP2 is secreted from lung myofibroblasts and is a potential biomarker for idiopathic pulmonary fibrosis Pubmed:30006483 |